|
| Status |
Public on Sep 01, 2011 |
| Title |
Gene expression in astrocytes upon sustained GSK-3 inhibition by small molecule |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to identify molecular targets that mediates the stimulation of astrocyte's migration upon sustained specific inhibition of GSK-3, we have employed whole genome microarray expression profiling. Murine cortical-striatal astrocytes grown in primary culture were treated in vitro for 48 h with either 1 micromolar Ro3303544, or control medium (final equivalent concentration of DMSO), or washed-out for 48h in control medium after an initial 48h treatment with 1 micromolar Ro3303544.
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| |
|
| Overall design |
The three different conditions: Ctrl, Ro and wash-out (each in duplicate) were compared.
|
| |
|
| Contributor(s) |
Renault-Mihara F, Okano H |
| Citation(s) |
21898827 |
| |
| Submission date |
Dec 02, 2010 |
| Last update date |
Jan 12, 2017 |
| Contact name |
francois frederic renault-mihara |
| E-mail(s) |
[email protected]
|
| Organization name |
Keio University School of Medicine
|
| Department |
Physiology
|
| Lab |
Physiology 1
|
| Street address |
35 Shinanomachi, Shinjuku-ku
|
| City |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
|
| Samples (6)
|
| GSM634052 |
control medium (10% FBS): replicate 1 |
| GSM634053 |
control medium (10% FBS): replicate 2 |
| GSM634054 |
treated 48h with 1 micromolar Ro3303544: replicate 1 |
| GSM634055 |
treated 48h with 1 micromolar Ro3303544: replicate 2 |
| GSM634056 |
washed-out for 48h after an initial 48 h treatment with 1 micromolar Ro3303544: replicate 1 |
| GSM634057 |
washed-out for 48h after an initial 48 h treatment with 1 micromolar Ro3303544: replicate 2 |
|
| Relations |
| BioProject |
PRJNA134075 |
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