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Series GSE25732 Query DataSets for GSE25732
Status Public on Jun 01, 2011
Title Expression data from Pou4f3-Rb1 cKO and control inner ear
Organism Mus musculus
Experiment type Expression profiling by array
Summary Retinoblastoma gene (Rb1) is required for proper cell cycle exit in the developing mouse inner ear and its deletion in the embryo leads to proliferation of sensory progenitor cells that differentiate into hair cells and supporting cells. In the Pou4f3-Cre:Rb1 flox/flox (Rb1 cKO) inner ear, utricular hair cells differentiate and survive into adulthood whereas differentiation and survival of cochlear hair cells are impaired. To comprehensively survey the pRb pathway in the mammalian inner ear, we performed microarray analysis of Rb1 cKO cochlea and utricle.
 
Overall design P6 or 2-month control and Rb1 cKO littermates were euthanized and the inner ear tissues were dissected. Total RNA was extracted from the pooled samples. Technical duplicates of the pooled RNA were used for microarray.
 
Contributor(s) Huang M, Chen Z
Citation(s) 21239885
Submission date Dec 01, 2010
Last update date Jan 08, 2019
Contact name Zheng-Yi Chen
E-mail(s) [email protected]
Phone 617-573-3673
Organization name MEEI
Department Otolaryngology
Lab Eaton Peabody Lab
Street address 243 Charles Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platforms (2)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
GPL340 [MOE430B] Affymetrix Mouse Expression 430B Array
Samples (24)
GSM632061 Ctrl-utr-2month-Technical-rep1-a
GSM632062 Ctrl-utr-2month-Technical-rep2-a
GSM632063 KO-utr-2month-Technical-rep1-a
Relations
BioProject PRJNA134077

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25732_RAW.tar 81.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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