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| Status |
Public on Nov 30, 2010 |
| Title |
Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
During early development, the correct establishment of the body axes is a critical step. The anterior pole of the mouse embryo is established when Distal Visceral Endoderm (DVE) cells migrate to form the Anterior Visceral Endoderm (AVE). Asymmetrical expression of Lefty1, Cerl and Dkk determines the direction of DVE migration and the future anterior side. Besides being implicated in the establishment of Anterior-Posterior axis the AVE has also been correlated with anterior neural specification. In order to better understand the role of the AVE in these processes, this cell population was isolated using a cerlP-EGFP transgenic mouse line, and a differential screening was performed using Affymetrix GeneChip technology. From this differential screening, 175 genes were found to be upregulated in the AVE, whereas 35 genes were upregulated in the Proximal-posterior sample. Using DAVID, here we characterize the AVE cell population regarding cellular component, molecular function and biological processes. Among the genes that were found to be upregulated in the AVE, several novel genes with expression in the AVE were identified. Four of the identified transcripts displaying high-fold change were further characterized by in situ hybridization in early stages of development in order to validate the screening. From those four selected genes, ADTK1 was chosen to be functionally characterized by targeted inactivation in ES cells. ADTK1 encodes for an unknown serine/threonine kinase. ADTK null mutants present short limbs and defects in the eye and ear. Taken together, these data point to the importance of reporting novel genes present in the AVE.
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| Overall design |
Anterior distal and Posterior proximal portions of an E5.5 mouse embryo were microdissected. RNA was extracted from this portions and hybridized on Affymetrix microarrays in order to identify genes upregulated in the Anterior distal sample. Whole E5.5 embryo RNA was also extracted and hybridized for normalization.
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| Contributor(s) |
Filipe M, Goncalves L, Becker JD, Belo JA |
| Citation(s) |
21553379 |
| |
| Submission date |
Nov 29, 2010 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Lisa Goncalves |
| E-mail(s) |
[email protected]
|
| Organization name |
CBME UALG
|
| Street address |
Campus de Gambelas, Ed8, Lab 1.17
|
| City |
Faro |
| ZIP/Postal code |
8005 |
| Country |
Portugal |
| |
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| Platforms (2) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
| GPL340 |
[MOE430B] Affymetrix Mouse Expression 430B Array |
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| Samples (10)
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| GSM630812 |
Anterior distal region of an E5.5 mouse embryo MOE430A rep1 |
| GSM630813 |
Anterior distal region of an E5.5 mouse embryo MOE430A rep2 |
| GSM630814 |
Posterior proximal region of an E5.5 mouse embryo MOE430A rep1 |
| GSM630815 |
Posterior proximal region of an E5.5 mouse embryo MOE430A rep2 |
| GSM630816 |
Whole E5.5 mouse embryo MOE430A rep1 |
| GSM630817 |
Anterior distal region of an E5.5 mouse embryo MOE430B rep3 |
| GSM630818 |
Anterior distal region of an E5.5 mouse embryo MOE430B rep4 |
| GSM630819 |
Posterior proximal region of an E5.5 mouse embryo MOE430B rep3 |
| GSM630820 |
Posterior proximal region of an E5.5 mouse embryo MOE430B rep4 |
| GSM630821 |
Whole E5.5 mouse embryo MOE430B rep1 |
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| Relations |
| BioProject |
PRJNA133871 |
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