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| Status |
Public on Jan 10, 2011 |
| Title |
Expression data from Human foreskin fibroblasts (HFFs) infected with Toxoplasma gondii. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Toxoplasma strains have been shown to modulate host cell transcription. We have found a type II Toxoplasma gene, GRA15, which activates the nuclear translocation of the NF-kappaB p65 transcription factor.
We used microarrays to determine how GRA15II modulates host cell transcription.
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| Overall design |
HFFs were infected with type I (RH), type I GRA15II (RH GRA15II), type II (Pru), type II GRA15KO (Pru GRA15KO), type III (cep), or type III GRA15II (cep GRA15II) parasites for 18-24 hours. Some samples were also stimulated with TNF-alpha. Total RNA was isolated and hybridized to Affymetrix GeneChip Human Genome U133A 2.0 arrays.
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| |
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| Contributor(s) |
Saeij JP, Rosowski EE, Julien L |
| Citation(s) |
21199955 |
| |
| Submission date |
Nov 18, 2010 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Emily E Rosowski |
| E-mail(s) |
[email protected]
|
| Organization name |
MIT
|
| Department |
Biology
|
| Lab |
Saeij
|
| Street address |
77 Massachusetts Ave, 68-258d
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (28)
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| This SubSeries is part of SuperSeries: |
| GSE25476 |
Expression data from host cells infected with different strains of Toxoplasma gondii |
|
| Relations |
| BioProject |
PRJNA142585 |
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