|
| Status |
Public on Aug 15, 2024 |
| Title |
Molecular mechanisms of BCAT1 in ASK120067-resistant NSCLC cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the mechanism of BCAT1 in mediating resistance to third-generation EGFR-TKI, we employed RNA-seq analysis to compare the transcriptome signature of ASK120067-resistant NSCLC cells with or without BCAT1 knockdown. Here, gene set enrichment (GSEA) revealed a significant decrease in the expression of genes in glycolysis pathway following small interfering RNA (siRNA)-mediated BCAT1 knockdown, which indicated the role for BCAT1 in supporting TKI resistance through upregulation of glycolysis.
|
| |
|
| Overall design |
Total mRNA profiles of 67R (ASK120067-resistant NCI-H1975) cells with and without BCAT1 knockdown.
|
| |
|
| Contributor(s) |
Zhang T, Pan Z, Xie H |
| Citation(s) |
39143065 |
| |
| Submission date |
Nov 13, 2023 |
| Last update date |
Aug 15, 2024 |
| Contact name |
Zilu Pan |
| E-mail(s) |
[email protected]
|
| Organization name |
Shanghai Institute of Materia Medica
|
| Street address |
No. 555 Zuchongzhi Road
|
| City |
Shanghai |
| ZIP/Postal code |
201203 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA1039771 |
Less...
More...