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| Status |
Public on Jan 05, 2012 |
| Title |
Gene regulation by phosphomimetic Myc and retinoic acid |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We aimed to investigate the E-box and Max-independent functions of the oncogene Myc in gene regulation and differentiation of leukemic cells. Expression in HL60 leukemic cells of a mutant form of Myc with impaired Max and E-box interaction by replacing the phosphorylation sites of Pak2 kinase to aspartic acid (MycD), resulted in downregulation of 235 genes and, interestingly, upregulation of 586 genes. Eleven percent of the genes upregulated by MycD coincided with those stimulated by a short treatment of retinoic acid (RA) alone. When leukemic cells expressing MycD were stimulated with RA, the overlap with the RA-alone signature increased to 32% (492/1556 genes). Importantly, 320 of these 492 bona-fide direct RA target genes were specifically superactivated in presence of MycD (MycD+RA>RA), and not by MycA (MycD+RA>MycA+RA), further suggesting a synergy between the two pathways. The list of superactivated genes included important regulators of development and differentiation such as GATA6, ICAM1 and HOX genes, whose expression was validated in independent experiments by RT-qPCR.
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| Overall design |
Induced gene expression in HL60 cells was measured after a short-treatment of retinoic acid (100nM, 4 hours). Cells were either wild-type or expressing ER-tagged MycD or MycA. Four biologically independent experiments were performed at each time.
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| Contributor(s) |
Uribesalgo I, Di Croce L, Roma G |
| Citation(s) |
22020439 |
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| Submission date |
Oct 15, 2010 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Luciano Di Croce |
| E-mail(s) |
[email protected]
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| Phone |
+34933160132
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| Organization name |
Centre for Genomic Regulation
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| Street address |
Dr. Aiguader, 88
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| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (18)
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| GSM609174 |
HL60 leukemic cells, empty vector, replicate 1 |
| GSM609175 |
HL60 leukemic cells, empty vector, replicate 2 |
| GSM609176 |
HL60 leukemic cells, empty vector, replicate 3 |
| GSM609177 |
HL60 leukemic cells, empty vector, replicate 4 |
| GSM609178 |
HL60 leukemic cells, MycD, replicate 1 |
| GSM609179 |
HL60 leukemic cells, MycD, replicate 2 |
| GSM609180 |
HL60 leukemic cells, MycD, replicate 3 |
| GSM609181 |
HL60 leukemic cells, MycD+RA, replicate 1 |
| GSM609182 |
HL60 leukemic cells, MycD+RA, replicate 2 |
| GSM609183 |
HL60 leukemic cells, MycD+RA, replicate 3 |
| GSM609184 |
HL60 leukemic cells, MycD+RA, replicate 4 |
| GSM609185 |
HL60 leukemic cells, RA, replicate 1 |
| GSM609186 |
HL60 leukemic cells, RA, replicate 2 |
| GSM609187 |
HL60 leukemic cells, RA, replicate 3 |
| GSM609188 |
HL60 leukemic cells, RA, replicate 4 |
| GSM609189 |
HL60 leukemic cells, MycA+RA, replicate 1 |
| GSM609190 |
HL60 leukemic cells, MycA+RA, replicate 2 |
| GSM609191 |
HL60 leukemic cells, MycA+RA, replicate 3 |
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| Relations |
| BioProject |
PRJNA132353 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24731_RAW.tar |
170.8 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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