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Status |
Public on Jun 28, 2024 |
Title |
Transcriptomic analysis of Drosophila leg disc associated myoblasts |
Organism |
Drosophila melanogaster |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Adult leg muscle precursors are associated to leg imaginal disc, here we provide bulk RNA sequecing of FACS sorted myoblasts from dissected imaginal leg discs at beginning of pupation.
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Overall design |
Drosophila leg discs were dissected from 0h after pupae formation. Leg disc cells were dissociated in collagenase and papain solution and cell-sorted using. RNA from GFP positive myoblasts and from control cells (whole leg disc cells) were extracted for analysis by deep sequencing, in triplicate, using Illumina NextSeq sequencing platform.
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Contributor(s) |
Moucaud B, Renaud Y, Soler C |
Citation(s) |
39374263 |
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Submission date |
Oct 12, 2023 |
Last update date |
Nov 22, 2024 |
Contact name |
yoan renaud |
E-mail(s) |
[email protected]
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Organization name |
igred
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Street address |
place henri dunand
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City |
clermont ferrand |
ZIP/Postal code |
63000 |
Country |
France |
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Platforms (1) |
GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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Samples (6)
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Relations |
BioProject |
PRJNA1027342 |