GEO Accession viewer

NCBI > GEO > Accession Display

Not logged in | Login

GEO help: Mouse over screen elements for information.

Series GSE2413

Query DataSets for GSE2413

Status

Public on Apr 15, 2005

Title

Timecourse of Gene Expression responses to cAMP in S49 Cells

Organism

Mus musculus

Experiment type

Expression profiling by array

Summary

Abstract

Although a substantial number of hormones and drugs increase cellular cAMP levels, the global impact of cAMP and its major effector mechanism, protein kinase A (PKA), on gene expression is not known. Here we show that treatment of wild-type S49 lymphoma cells for 24 h with 8-(4-chlorophenylthio)-cAMP (CPT-cAMP), a PKA-selective cAMP analog, alters the expression of ~4500 of ~13,600 unique genes. By contrast, gene expression was unaltered in Kin– S49 cells (that lack PKA) incubated with CPT-cAMP. Changes in mRNA and protein expression of several cell-cycle regulators accompanied cAMP-induced G1-phase cell-cycle arrest of wild-type S49 cells. Within 2 h, CPT-cAMP altered expression of 152 genes that contain evolutionarily conserved cAMP-response elements (CRE) within 5 kb of transcriptional start sites, including the circadian clock gene Per1. Thus, cAMP through its activation of PKA produces extensive transcriptional regulation in eukaryotic cells. These transcriptional networks include a primary group of CRE-containing genes and secondary networks that include the circadian clock.

Keywords: time-course

Contributor(s)

Zambon AC, Zhang L, Minovitsky S, Kanter J, Prabhakar S, Salomonis N, Vranizan K, Dubchak I, Conklin BR, Insel PA

Citation(s)

15939874

Submission date

Mar 16, 2005

Last update date

Jan 08, 2019

Contact name

Alexander C Zambon

Organization name

Keck Graduate Institute