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| Status |
Public on Sep 02, 2010 |
| Title |
Polyphenols from Myrothamnus flabellifolia Welw. inhibit in vitro adhesion of Porphyromonas gingivalis and exert anti-inflammatory cytoprotective effects in KB cells |
| Platform organisms |
Streptococcus mutans UA159; Porphyromonas gingivalis W83 |
| Sample organism |
Porphyromonas gingivalis ATCC 33277 |
| Experiment type |
Expression profiling by array
|
| Summary |
Investigation of whole genome gene expression level changes in Porphyromonas gingivalis ATCC 33277 treated with an anti-adhesive extract from Myrothamnus flabellifolia compared to the untreated strain.
Aim: Identification of anti-adhesive plant extracts against cell surface binding of Porphyromonas gingivalis.
Materials and Methods: Polyphenol-enriched extract from Myrothamnus flabellifolia (MF) traditionally used for periodontitis was tested for inhibition of P. gingivalis adhesion to KB cells by FACS, for influence on gingipain activity, hemagglutination and by microarray analysis for effects on the bacterial transcriptome. P. gingivalis-induced inflammation parameters were monitored by RT-PCR.
Results: MF (100 µg/ml) reduced P. gingivalis adhesion/invasion about 50% by interacting with fimbriae and bacterial OMPs. Microarray analysis of MF-treated bacteria indicated up-regulation of genes involved in cell adhesion. As confirmed by RT-PCR, fimbrillin- and Arg-gingipain-encoding genes were upregulated by MF. On the protein level, inhibition (70%) of Arg-gingipain activity was observed, while the corresponding Lys-gingipain was hardly influenced. MF also inhibited hemagglutination. While exposure to P. gingivalis resulted in an increased expression of inflammation-related genes in KB cells, pretreatment of KB cells with MF evoked cytoprotective effects concerning IL-1β, IL-6, IL-8 and TNFα gene expression as well as IL-6 release rates.
Conclusions: While being cytoprotective, MF exerts strong anti-adhesive effects against P. gingivalis. Thus, MF may be useful for the prevention of P. gingivalis-associated periodontal diseases.
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| Overall design |
The chip study used total RNA recovered from two separate MF-treated and two separate untreated Porphyromonas gingivalis ATCC 33277 cultures. Each chip measured the expression level of 1,842 genes from P. gingivalis ATCC 33277 with thirteen 60-mer probes per gene, with three-fold technical redundancy.
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| Contributor(s) |
Löhr G, Beikler T, Podbielski A, Standar K, Redanz S, Hensel A |
| Citation(s) |
21158896 |
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| Submission date |
Sep 01, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Sylvio Redanz |
| E-mail(s) |
[email protected]
|
| Phone |
0381 494 5939
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| Organization name |
hospital university rostock
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| Department |
Institute of microbiology, virology and hygiene
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| Lab |
medical microbiology
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| Street address |
Schillingallee 70
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| City |
Rostock |
| State/province |
Mecklenburg Vorpommern |
| ZIP/Postal code |
18057 |
| Country |
Germany |
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| Platforms (1) |
| GPL10873 |
NimbleGen Porphyromonas gingivalis Streptococcus mutans 080319_BK_mixed_bact_expr |
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| Samples (2) |
| GSM590017 |
P. gingivalis ATCC 33277, untreated |
| GSM590018 |
P. gingivalis ATCC 33277, MF-treated |
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| Relations |
| BioProject |
PRJNA130317 |
