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| Status |
Public on Aug 18, 2010 |
| Title |
Comparison of Expression profile between CD3-CD56dimCD16+ CD57+ and CD57neg NK cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Thirty to 60% of CD56dimCD16bright NK cells in healthy adults express CD57, which is not expressed on immature CD56bright NK cells or fetal and newborn NK cells. We hypothesized that CD57+ NK cells within the CD56dim mature NK cell subset are highly mature and might be terminally differentiated.
We used microarrays to assess the transcriptional differences between CD57+ and CD57neg NK cells within the CD56dim mature NK subset.
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| Overall design |
PBMC were obtained from leukocyte reduction Pall filters (Blood Centers of the Pacific) after density gradient centrifugation over Ficoll-Paque (GE Healthcare). CD57+CD3negCD56dimCD16+ and CD57negCD3negCD56dimCD16+ NK cells from three healthy adult donors were sorted into Lysis Solution (100 ml per 10,000 cells) (RNAqueous-Micro kit, Ambion). RNA was analyzed by BioAnalyzer (Agilent) before amplification with the NuGEN WT-Ovation kit (Roche) and analyzing transcripts using Affymetrix Human Gene 1.0 ST arrays. Moderated paired t-tests were used to identify differentially expressed genes (>1.7-fold different) between CD57+ and CD57neg NK cells. The P values were set at <0.001 and <0.005.
Supplementary table below reports Fold difference and M data.
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| Contributor(s) |
Lopez-Verges S, Lanier LL |
| Citation(s) |
20733159 |
| |
| Submission date |
Aug 18, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Sandra Laurence Lopez-Verges |
| E-mail(s) |
[email protected]
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| Organization name |
University of California San Francisco
|
| Department |
Microbiology and Immunology
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| Lab |
Lewis Lanier lab
|
| Street address |
513 Parnassus Ave, Room HSE 1001G
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143-0414 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM581225 |
CD57+NK cell, donor 54, biological rep1 |
| GSM581226 |
CD57negNK cell, donor 54, biological rep1 |
| GSM581227 |
CD57+NK cell, donor 55, biological rep2 |
| GSM581229 |
CD57negNK cell, donor 55, biological rep2 |
| GSM581230 |
CD57+NK cell, donor 56, biological rep3 |
| GSM581232 |
CD57negNK cell, donor 56, biological rep3 |
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| Relations |
| BioProject |
PRJNA130867 |
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