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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 25, 2023 |
| Title |
Germline piRNAs counteract endogenous retrovirus invasion from somatic cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In metazoan gonads, transposable elements (TEs) mobilization is limited by PIWI-interacting RNAs (piRNAs). These small RNAs originate from specific source loci, the piRNA clusters. piRNAs are known to silence TEs in the cells where they are produced. Endogenous retroviruses (ERVs), a subclass of TEs, pose a particular threat because they are capable of transiting from cell to cell. In this study, we reveal that piRNAs produced locally in germ cells counteract invasion by ERVs arriving from adjacent somatic cells. We reactivated the Drosophila ERVZAMin somatic gonadal cells by deleting, using CRISPR-Cas9 genome editing, its single copy in the somaticflamencopiRNA cluster, while keeping the piRNA pathway fully functional. Upon reactivation,ZAMinvaded the oocytes, resulting in transposition and severe fertility defects. We show that onceZAM-piRNAs are produced in germ cells they counter the invasion. Our study sheds new light on the mechanisms of recognition and regulation of invasive genetic elements, which is essential for the maintenance of genome integrity.
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| Overall design |
RNA sequencing
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| Contributor(s) |
Yoth M, Guegen N, Jensen S, Brasset E |
| Citation(s) |
37773253 |
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| Submission date |
Jun 27, 2023 |
| Last update date |
Oct 18, 2023 |
| Contact name |
Emilie BRASSET |
| E-mail(s) |
[email protected]
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| Organization name |
CNRS 6293 INSERM U1103 Université Clermont Auvergne
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| Lab |
iGReD
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| Street address |
28 place Henri dunant
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| City |
CLERMONT-FERRAND |
| ZIP/Postal code |
63000 |
| Country |
France |
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| Platforms (1) |
| GPL32218 |
DNBSEQ-G400 (Drosophila melanogaster) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA988107 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE235966_RAW.tar |
199.4 Mb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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