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| Status |
Public on Jun 20, 2023 |
| Title |
Assessment of the molecular mechanism associated with osteogenic and angiogenic differentiation induced by FeMnSi scaffold |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the molecular mechanism underlying osteogenic and angiogenic differentiation induced by the De-HA scaffold, we conducted an in-depth gene microarray analysis on day 3 of MC3T3-E1 culture. We found that De-HA could enhance the transcription of osteogenic and angiogenic genes by activating FAK and downstream MAPK/ERK signaling to induce MC3T3-E1 differentiation. More importantly, our findings indicated that EGFR, a kind of transmembrane glycoprotein, played a key role in the osteogenic differentiation of MC3T3-E1, suggesting that the activation of the FAK/MAPK/ERK signaling cascade might be driven by EGFR.
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| Overall design |
The scaffold samples were segregated into two groups: the first comprised of the original FeMnSi scaffold, while the second consisted of a De-HA scaffold that had been dealloyed and coated with bioactive materials. MC3T3-E1 were seeded on each scaffold at a density of 1 × 105 per/well in 24-well plates and then cultured for 3 days. An extensive gene microarray analysis was conducted on day 3 of MC3T3-E1 culture.
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| Contributor(s) |
Yuan B, Chen Z |
| Citation missing |
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| Submission date |
Jun 16, 2023 |
| Last update date |
Jun 23, 2023 |
| Contact name |
Bo Yuan |
| E-mail(s) |
[email protected]
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| Organization name |
Sichuan University
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| Street address |
Jiuyanqiao Wangjiang Road
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| City |
Chengdu |
| ZIP/Postal code |
610064 |
| Country |
China |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA984698 |
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