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| Status |
Public on Jul 22, 2024 |
| Title |
Innate immune memory after brain injury drives inflammatory cardiac dysfunction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Besides the local inflammatory immune response in the brain, stroke also alters systemic immunity. Within the acute and sub-acute phase, the systemic immune response to stroke has been described in detail over last decades. The long-term systemic immunological consequences after stroke are however still elusive. Therefore, a better understanding of these long-lasting chronic effects of stroke on systemic immunity and its impact on remote organ function and secondary comorbidities is needed. Here, we used single-cell RNA sequencing (scRNA-Seq) to investigate the chronic effect of stroke on the transcriptomic signatures of resident myeloid immune cells in various peripheral organs remote from the brain, including the lung, heart, liver, spleen, blood and bone marrow. We observed that monocytes in peripheral organs and in the bone marrow adopt a pro-inflammatory phenotype which persists chronically after stroke. Moreover, the pro-inflammatory profile of monocytes in the bone marrow was transmissible by BM transplantation to naïve recipients, indicating potentially epigenetically imprinted chronic innate immune memory after stroke. Indeed, by performing single-nuclei ATAC sequencing, we found that post-stroke myeloid immune memory after stroke is likely mediated by IL-1b-driven mechanisms, and that neutralization of the post-stroke increase in circulating IL-1b prevented myeloid immune memory.
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| Overall design |
Three independent mouse single-cell RNA experiments were performed on myeloid cells from different peripheral organs (blood, spleen, heart, lung, liver) and bone marrow, chronically after stroke and control conditions.
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| Web link |
https://doi.org/10.1016/j.cell.2024.06.028
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| Contributor(s) |
Simats A, Zhang S, Messerer D, Chong F, Beskardes S, Chivukula A, Cao J, Besson-Girard S, Montellanos F, Morbach C, Carofiglio O, Ricci A, Roth S, Llovera G, Singh R, Chen Y, Filser S, Plesnila N, Braun C, Spitzer H, Gocke O, Dichgans M, Heuschmann P, Hatakeyama K, Beltrán E, Clauss S, Bonev B, Schulz C, Liesz A |
| Citation(s) |
39043180 |
| |
| Submission date |
May 09, 2023 |
| Last update date |
Oct 22, 2024 |
| Contact name |
Arthur Liesz |
| E-mail(s) |
[email protected]
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| Organization name |
LMU Hospital
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| Department |
Institute for Stroke and Dementia (ISD)
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| Street address |
Feodor-Lynen-Straße 17
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| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (20)
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| GSM7312143 |
Peripheral organs, Control, scRNAseq, rep1 |
| GSM7312144 |
Peripheral organs, Stroke, scRNAseq, rep1 |
| GSM7312145 |
Peripheral organs, Control, scRNAseq, rep2 |
| GSM7312146 |
Peripheral organs, Stroke, scRNAseq,rep2 |
| GSM7312147 |
Peripheral organs, Control, scRNAseq, rep3 |
| GSM7312148 |
Peripheral organs, Stroke, scRNAseq, rep3 |
| GSM7312149 |
Peripheral organs, Control, scRNAseq, rep4 |
| GSM7312150 |
Peripheral organs, Stroke, scRNAseq, rep4 |
| GSM7312151 |
Bone marrow, Control, ScRNAseq, rep1 |
| GSM7312152 |
Bone marrow, Stroke, ScRNAseq, rep1 |
| GSM7312153 |
Bone marrow, Control, ScRNAseq, rep2 |
| GSM7312154 |
Bone marrow, Stroke, ScRNAseq, rep2 |
| GSM7312155 |
Bone marrow, Control, ScRNAseq, rep3 |
| GSM7312156 |
Bone marrow, Stroke, ScRNAseq, rep3 |
| GSM7312157 |
Bone marrow, Control, ScRNAseq, rep4 |
| GSM7312158 |
Bone marrow, Stroke, ScRNAseq, rep4 |
| GSM7312159 |
Recipient bone marrow, Control donor, ScRNAseq, rep1 |
| GSM7312160 |
Recipient bone marrow, Stroke donor, ScRNAseq, rep1 |
| GSM7312161 |
Recipient bone marrow, Control donor, ScRNAseq, rep2 |
| GSM7312162 |
Recipient bone marrow, Stroke donor, ScRNAseq, rep2 |
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| Relations |
| BioProject |
PRJNA970816 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE232098_BM_barcodes.tsv.gz |
157.6 Kb |
(ftp)(http) |
TSV |
| GSE232098_BM_features.tsv.gz |
272.8 Kb |
(ftp)(http) |
TSV |
| GSE232098_BM_matrix.mtx.gz |
279.3 Mb |
(ftp)(http) |
MTX |
| GSE232098_BMtransplant_barcodes.tsv.gz |
223.8 Kb |
(ftp)(http) |
TSV |
| GSE232098_BMtransplant_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
| GSE232098_BMtransplant_matrix.mtx.gz |
486.3 Mb |
(ftp)(http) |
MTX |
| GSE232098_peripheral_barcodes.tsv.gz |
216.1 Kb |
(ftp)(http) |
TSV |
| GSE232098_peripheral_features.tsv.gz |
272.9 Kb |
(ftp)(http) |
TSV |
| GSE232098_peripheral_matrix.mtx.gz |
302.1 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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