|
| Status |
Public on Jul 27, 2023 |
| Title |
Transcription factor cooperation and competition |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Transcription factors have to distinguish proper targets from a large pool of potential binding sites. Here we explore how MSL2 and CLAMP cooperate to bind their specific targets. Using an in vitro chromatin reconstitution system and by mutating the interaction domain of CLAMP we show how direct and indirect cooperation of TF binding influence binding specificity.
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| |
|
| Overall design |
ChiP-seq profiles of MSL2 CLAMP and CLAMP mutant in vitro reconstituted chromatin
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| |
|
| Contributor(s) |
Eggers N |
| Citation(s) |
37602401 |
| |
| Submission date |
Feb 09, 2023 |
| Last update date |
Sep 27, 2023 |
| Contact name |
Nikolas Eggers |
| E-mail(s) |
[email protected]
|
| Organization name |
LMU Munich
|
| Department |
Molecular Biology
|
| Lab |
Becker
|
| Street address |
Grosshaderner Strasse 9
|
| City |
Munich |
| ZIP/Postal code |
80469 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL33093 |
NextSeq 1000 (Drosophila melanogaster) |
|
| Samples (28)
|
|
| Relations |
| BioProject |
PRJNA933216 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE224981_merged.chip_clamp.ipnorm.bw |
100.0 Mb |
(ftp)(http) |
BW |
| GSE224981_merged.chip_clamp.peaks.bed.gz |
1.7 Kb |
(ftp)(http) |
BED |
| GSE224981_merged.chip_clamp_msl2.ipnorm.bw |
101.6 Mb |
(ftp)(http) |
BW |
| GSE224981_merged.chip_clamp_msl2.peaks.bed.gz |
6.0 Kb |
(ftp)(http) |
BED |
| GSE224981_merged.chip_clamp_mut.ipnorm.bw |
91.6 Mb |
(ftp)(http) |
BW |
| GSE224981_merged.chip_clamp_mut.peaks.bed.gz |
1.3 Kb |
(ftp)(http) |
BED |
| GSE224981_merged.chip_clamp_mut_msl2.ipnorm.bw |
97.0 Mb |
(ftp)(http) |
BW |
| GSE224981_merged.chip_clamp_mut_msl2.peaks.bed.gz |
1.7 Kb |
(ftp)(http) |
BED |
| GSE224981_merged.chip_flag_clamp.ipnorm.bw |
231.2 Mb |
(ftp)(http) |
BW |
| GSE224981_merged.chip_flag_clamp.peaks.bed.gz |
5.0 Kb |
(ftp)(http) |
BED |
| GSE224981_merged.chip_flag_clamp_mut.ipnorm.bw |
242.0 Mb |
(ftp)(http) |
BW |
| GSE224981_merged.chip_flag_clamp_mut.peaks.bed.gz |
3.5 Kb |
(ftp)(http) |
BED |
| GSE224981_merged.chip_msl2.ipnorm.bw |
225.2 Mb |
(ftp)(http) |
BW |
| GSE224981_merged.chip_msl2.peaks.bed.gz |
2.4 Kb |
(ftp)(http) |
BED |
| GSE224981_merged.chip_msl2_clamp.ipnorm.bw |
197.0 Mb |
(ftp)(http) |
BW |
| GSE224981_merged.chip_msl2_clamp.peaks.bed.gz |
6.3 Kb |
(ftp)(http) |
BED |
| GSE224981_merged.chip_msl2_clamp_mut.ipnorm.bw |
207.6 Mb |
(ftp)(http) |
BW |
| GSE224981_merged.chip_msl2_clamp_mut.peaks.bed.gz |
3.6 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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