|
| Status |
Public on Feb 15, 2023 |
| Title |
Fluorescence detection of FAO activity with a chemical probe in cultured cells and mouse liver tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Fatty acid beta oxidation (FAO) pathway is an important metabolic pathway for energy generation and is deeply associated with various diseases such as cancer and NAFLD/NASH. Therefore, development of detection methods of FAO activity change would contribute to understanding of disease mechanism and drug discovery. In this study, we newly developed a fluorescent probe for fluorescence detection of FAO activity in cultured cells and mouse liver tissue and applied this probe for investigation of the heterogeneity of FAO activity in mouse liver tissue by FACS and mRNA sequence.
|
| |
|
| Overall design |
Hepatocytes in the liver of the mice administrated with our fluorescent probe were separated by FACS and mRNAs expressing in the hepatocytes were investigated for understanding of heterogeneity of FAO activity.
|
| |
|
| Contributor(s) |
Matsunaga N, Yoshida Y, Ojida A, Uchinomya S, Hagiwara H, Yasuda K, Doi A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 06, 2023 |
| Last update date |
Feb 15, 2023 |
| Contact name |
Yuya Yoshida |
| E-mail(s) |
[email protected]
|
| Phone |
0926426658
|
| Organization name |
Kyushu-university
|
| Street address |
東区馬出3-1-1 九州大学薬学部棟本館3F 薬剤学教室, 薬物動態学分野
|
| City |
福岡市 |
| State/province |
福岡県 |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platforms (1) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA932004 |
Less...
More...