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| Status |
Public on Feb 08, 2023 |
| Title |
Molecular Logic of Synaptic Diversity Between Drosophila Tonic and Phasic Motoneurons |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Although neuronal subtypes often display unique synaptic organization and function, the underlying transcriptional differences necessary to establish these features is poorly understood. To identify molecular pathways that establish synaptic diversity, single neuron PatchSeq RNA profiling was performed on Drosophila tonic and phasic glutamatergic motoneurons. Tonic motoneurons form weaker facilitating synapses onto single muscles, while phasic motoneurons form stronger depressing synapses onto multiple muscles. Super-resolution microscopy and in vivo imaging demonstrated synaptic active zones in phasic motoneurons are more compact and display enhanced Ca2+ influx compared to their tonic counterparts. Genetic analysis identified unique synaptic properties that mapped onto gene expression differences in several cellular pathways, including distinct signaling ligands, post-translational modifications and intracellular Ca2+ buffers. These findings provide insights into how unique transcriptomes can drive functional and morphological differences between two closely related neuronal subgroups.
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| Overall design |
PatchSeq RNA profiling of 105 1B motor neurons, 101 1S motor neurons, 17 M1 muscle cells and 26 M4 muscle cells.
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| |
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| Contributor(s) |
Jetti SK, Crane AB, Akbergenova Y, Aponte-Santiago NA, Cunningham KL, Whittaker CA, Littleton JT |
| Citation(s) |
36711745, 37717212 |
| |
| Submission date |
Jan 16, 2023 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Charles Arthur Whittaker |
| E-mail(s) |
[email protected]
|
| Organization name |
Koch Institute
|
| Street address |
77 Mass Ave 76-189
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02152 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
|
| Samples (249)
|
| GSM6936130 |
1S neuron, seqment A3, animal 1, cell d225001 |
| GSM6936131 |
1S neuron, seqment A2, animal 1, cell d225002 |
| GSM6936132 |
1B neuron, seqment A2, animal 2, cell d225003 |
| GSM6936133 |
1B neuron, seqment A5, animal 2, cell d225005 |
| GSM6936134 |
1B neuron, seqment A3, animal 3, cell d225006 |
| GSM6936135 |
1B neuron, seqment A5, animal 3, cell d225007 |
| GSM6936136 |
1B neuron, seqment A6, animal 3, cell d225008 |
| GSM6936137 |
1B neuron, seqment A7, animal 3, cell d225009 |
| GSM6936138 |
1B neuron, seqment A8, animal 3, cell d225010 |
| GSM6936139 |
1B neuron, seqment A8, animal 4, cell d225011 |
| GSM6936140 |
1S neuron, seqment A2, animal 4, cell d225012 |
| GSM6936141 |
1S neuron, seqment A3, animal 4, cell d225013 |
| GSM6936142 |
1S neuron, seqment A3, animal 4, cell d225014 |
| GSM6936143 |
1S neuron, seqment A2, animal 4, cell d225015 |
| GSM6936144 |
1S neuron, seqment A6, animal 4, cell d225016 |
| GSM6936145 |
1S neuron, seqment A2, animal 4, cell d225017 |
| GSM6936146 |
1S neuron, seqment A6, animal 4, cell d225018 |
| GSM6936147 |
1S neuron, seqment A4, animal 4, cell d225019 |
| GSM6936148 |
1S neuron, seqment A1, animal 4, cell d225020 |
| GSM6936149 |
1S neuron, seqment A1, animal 4, cell d225021 |
| GSM6936150 |
1B neuron, seqment A2 , animal 5, cell d225022 |
| GSM6936151 |
1B neuron, seqment A5, animal 5, cell d225023 |
| GSM6936152 |
1B neuron, seqment A3, animal 5, cell d225024 |
| GSM6936153 |
1B neuron, seqment A5, animal 5, cell d225025 |
| GSM6936154 |
1B neuron, seqment A6, animal 5, cell d225026 |
| GSM6936155 |
1B neuron, seqment A2, animal 5, cell d225027 |
| GSM6936156 |
1B neuron, seqment T3, animal 5, cell d225028 |
| GSM6936157 |
1B neuron, seqment T2, animal 5, cell d225029 |
| GSM6936158 |
1B neuron, seqment A8, animal 5, cell d225030 |
| GSM6936159 |
1B neuron, seqment A2, animal 5, cell d225031 |
| GSM6936160 |
1S neuron, seqment A4, animal 6, cell d225032 |
| GSM6936161 |
1S neuron, seqment A2, animal 6, cell d225033 |
| GSM6936162 |
1S neuron, seqment T2, animal 6, cell d225034 |
| GSM6936163 |
1S neuron, seqment T2, animal 6, cell d225035 |
| GSM6936164 |
1S neuron, seqment A5, animal 6, cell d225036 |
| GSM6936165 |
1S neuron, seqment A8, animal 6, cell d225037 |
| GSM6936166 |
1S neuron, seqment A8, animal 6, cell d225039 |
| GSM6936167 |
1S neuron, seqment A7, animal 6, cell d225040 |
| GSM6936168 |
1B neuron, seqment A1, animal 7, cell d225041 |
| GSM6936169 |
1B neuron, seqment A2, animal 7, cell d225042 |
| GSM6936170 |
1B neuron, seqment T3, animal 7, cell d225043 |
| GSM6936171 |
1B neuron, seqment A3, animal 7, cell d225045 |
| GSM6936172 |
1B neuron, seqment A2, animal 7, cell d225047 |
| GSM6936173 |
1B neuron, seqment A6, animal 7, cell d225048 |
| GSM6936174 |
1B neuron, seqment A7, animal 7, cell d225049 |
| GSM6936175 |
1B neuron, seqment T3, animal 7, cell d225050 |
| GSM6936176 |
1B neuron, seqment A7, animal 7, cell d225051 |
| GSM6936177 |
1B neuron, seqment A6, animal 7, cell d225052 |
| GSM6936178 |
1S neuron, seqment A1, animal 8, cell d225053 |
| GSM6936179 |
1S neuron, seqment A7, animal 8, cell d225054 |
| GSM6936180 |
1S neuron, seqment A5, animal 8, cell d225055 |
| GSM6936181 |
1S neuron, seqment A7, animal 8, cell d225056 |
| GSM6936182 |
1S neuron, seqment A5, animal 8, cell d225057 |
| GSM6936183 |
1S neuron, seqment T1, animal 8, cell d225058 |
| GSM6936184 |
1B neuron, seqment A1, animal 9, cell d225059 |
| GSM6936185 |
1B neuron, seqment A2, animal 9, cell d225060 |
| GSM6936186 |
1B neuron, seqment A3, animal 9, cell d225061 |
| GSM6936187 |
1B neuron, seqment A3, animal 9, cell d225062 |
| GSM6936188 |
1B neuron, seqment A1, animal 9, cell d225063 |
| GSM6936189 |
1B neuron, seqment T1, animal 9, cell d225064 |
| GSM6936190 |
1B neuron, seqment A5, animal 9, cell d225065 |
| GSM6936191 |
1B neuron, seqment A7, animal 9, cell d225066 |
| GSM6936192 |
1B neuron, seqment A7, animal 9, cell d225067 |
| GSM6936193 |
1S neuron, seqment A2, animal 10, cell d225068 |
| GSM6936194 |
1S neuron, seqment A5, animal 10, cell d225069 |
| GSM6936195 |
1S neuron, seqment A1, animal 10, cell d225070 |
| GSM6936196 |
1S neuron, seqment A2, animal 10, cell d225071 |
| GSM6936197 |
1S neuron, seqment A2, animal 11, cell d225072 |
| GSM6936198 |
1S neuron, seqment A3, animal 11, cell d225074 |
| GSM6936199 |
1S neuron, seqment A4, animal 11, cell d225075 |
| GSM6936200 |
1S neuron, seqment A5, animal 11, cell d225076 |
| GSM6936201 |
1S neuron, seqment A6, animal 11, cell d225077 |
| GSM6936202 |
1S neuron, seqment A7, animal 11, cell d225078 |
| GSM6936203 |
1S neuron, seqment T1, animal 11, cell d225079 |
| GSM6936204 |
1S neuron, seqment A8, animal 11, cell d225080 |
| GSM6936205 |
1S neuron, seqment A7, animal 11, cell d225081 |
| GSM6936206 |
1S neuron, seqment A5, animal 11, cell d225082 |
| GSM6936207 |
1B neuron, seqment A5, animal 12, cell d225084 |
| GSM6936208 |
1B neuron, seqment A3, animal 12, cell d225085 |
| GSM6936209 |
1B neuron, seqment A3, animal 12, cell d225087 |
| GSM6936210 |
1B neuron, seqment A4, animal 12, cell d225088 |
| GSM6936211 |
1B neuron, seqment A7, animal 12, cell d225089 |
| GSM6936212 |
1B neuron, seqment A8, animal 12, cell d225090 |
| GSM6936213 |
1B neuron, seqment A7, animal 12, cell d225091 |
| GSM6936214 |
1B neuron, seqment A5, animal 12, cell d225094 |
| GSM6936215 |
1B neuron, seqment A6, animal 12, cell d225095 |
| GSM6936216 |
1S neuron, seqment A4, animal 1, cell d270001 |
| GSM6936217 |
1S neuron, seqment A5, animal 1, cell d270003 |
| GSM6936218 |
1S neuron, seqment A6, animal 1, cell d270004 |
| GSM6936219 |
1S neuron, seqment A2, animal 2, cell d270005 |
| GSM6936220 |
1S neuron, seqment A4, animal 2, cell d270006 |
| GSM6936221 |
1S neuron, seqment A1, animal 2, cell d270007 |
| GSM6936222 |
1S neuron, seqment A3, animal 2, cell d270008 |
| GSM6936223 |
1S neuron, seqment A8, animal 2, cell d270009 |
| GSM6936224 |
1S neuron, seqment A7, animal 2, cell d270010 |
| GSM6936225 |
1S neuron, seqment A7, animal 2, cell d270012 |
| GSM6936226 |
1S neuron, seqment A6, animal 2, cell d270013 |
| GSM6936227 |
1B neuron, seqment A7, animal 3, cell d270014 |
| GSM6936228 |
1B neuron, seqment A5, animal 3, cell d270016 |
| GSM6936229 |
1B neuron, seqment A6, animal 3, cell d270017 |
| GSM6936230 |
1B neuron, seqment A3, animal 3, cell d270018 |
| GSM6936231 |
1S neuron, seqment A5, animal 4, cell d270022 |
| GSM6936232 |
1S neuron, seqment A7, animal 4, cell d270023 |
| GSM6936233 |
1S neuron, seqment A6, animal 4, cell d270024 |
| GSM6936234 |
1B neuron, seqment A3, animal 5, cell d270026 |
| GSM6936235 |
1B neuron, seqment A5, animal 5, cell d270027 |
| GSM6936236 |
1B neuron, seqment A8, animal 5, cell d270029 |
| GSM6936237 |
1B neuron, seqment A8, animal 5, cell d270030 |
| GSM6936238 |
1S neuron, seqment A2, animal 4, cell d270031 |
| GSM6936239 |
1S neuron, seqment A3, animal 4, cell d270032 |
| GSM6936240 |
1B neuron, seqment A5, animal 6, cell d270034 |
| GSM6936241 |
1B neuron, seqment A5, animal 6, cell d270035 |
| GSM6936242 |
1B neuron, seqment A6, animal 6, cell d270036 |
| GSM6936243 |
1B neuron, seqment A6, animal 6, cell d270037 |
| GSM6936244 |
1B neuron, seqment A7, animal 6, cell d270038 |
| GSM6936245 |
1S neuron, seqment A1, animal 7, cell d270040 |
| GSM6936246 |
1S neuron, seqment A2, animal 7, cell d270041 |
| GSM6936247 |
1S neuron, seqment A4, animal 7, cell d270042 |
| GSM6936248 |
1S neuron, seqment A6, animal 7, cell d270043 |
| GSM6936249 |
1S neuron, seqment T3, animal 7, cell d270044 |
| GSM6936250 |
1S neuron, seqment T2, animal 7, cell d270045 |
| GSM6936251 |
1S neuron, seqment A7, animal 7, cell d270047 |
| GSM6936252 |
1S neuron, seqment A6, animal 7, cell d270048 |
| GSM6936253 |
1B neuron, seqment A2, animal 8, cell d270049 |
| GSM6936254 |
1B neuron, seqment A4, animal 8, cell d270050 |
| GSM6936255 |
1B neuron, seqment A6, animal 8, cell d270051 |
| GSM6936256 |
1B neuron, seqment A6, animal 9, cell d270052 |
| GSM6936257 |
1B neuron, seqment A8, animal 9, cell d270053 |
| GSM6936258 |
1B neuron, seqment A7, animal 9, cell d270054 |
| GSM6936259 |
1B neuron, seqment A6, animal 9, cell d270055 |
| GSM6936260 |
1S neuron, seqment A3, animal 10, cell d270059 |
| GSM6936261 |
1S neuron, seqment A2, animal 10, cell d270060 |
| GSM6936262 |
1S neuron, seqment A2, animal 10, cell d270061 |
| GSM6936263 |
1S neuron, seqment A7, animal 10, cell d270062 |
| GSM6936264 |
1S neuron, seqment A8, animal 10, cell d270063 |
| GSM6936265 |
1S neuron, seqment A7, animal 10, cell d270064 |
| GSM6936266 |
1S neuron, seqment A6, animal 10, cell d270065 |
| GSM6936267 |
1S neuron, seqment A6, animal 10, cell d270066 |
| GSM6936268 |
1S neuron, seqment A4, animal 10, cell d270067 |
| GSM6936269 |
1S neuron, seqment A3, animal 10, cell d270068 |
| GSM6936270 |
1S neuron, seqment A1, animal 10, cell d270069 |
| GSM6936271 |
1B neuron, seqment A6, animal 11, cell d270075 |
| GSM6936272 |
1B neuron, seqment A6, animal 11, cell d270077 |
| GSM6936273 |
1B neuron, seqment A7, animal 11, cell d270078 |
| GSM6936274 |
1B neuron, seqment A5, animal 11, cell d270079 |
| GSM6936275 |
1B neuron, seqment A4, animal 11, cell d270080 |
| GSM6936276 |
1B neuron, seqment A2, animal 11, cell d270081 |
| GSM6936277 |
1B neuron, seqment A3, animal 11, cell d270082 |
| GSM6936278 |
1B neuron, seqment A7, animal 12, cell d270088 |
| GSM6936279 |
1B neuron, seqment A6, animal 12, cell d270089 |
| GSM6936280 |
1B neuron, seqment A4, animal 12, cell d270090 |
| GSM6936281 |
1B neuron, seqment A3, animal 12, cell d270091 |
| GSM6936282 |
1B neuron, seqment A2, animal 12, cell d270092 |
| GSM6936283 |
1B neuron, seqment A2, animal 12, cell d270094 |
| GSM6936284 |
1B neuron, seqment A3, animal 12, cell d270096 |
| GSM6936285 |
1B neuron, seqment A5, animal 1, cell d306001 |
| GSM6936286 |
1B neuron, seqment A5, animal 1, cell d306002 |
| GSM6936287 |
1B neuron, seqment A4, animal 1, cell d306003 |
| GSM6936288 |
1B neuron, seqment A4, animal 1, cell d306004 |
| GSM6936289 |
1B neuron, seqment A6, animal 1, cell d306005 |
| GSM6936290 |
1B neuron, seqment A7, animal 1, cell d306006 |
| GSM6936291 |
1S neuron, seqment A5, animal 3, cell d306009 |
| GSM6936292 |
1S neuron, seqment A7, animal 3, cell d306010 |
| GSM6936293 |
1S neuron, seqment A4, animal 3, cell d306011 |
| GSM6936294 |
1S neuron, seqment A3, animal 3, cell d306012 |
| GSM6936295 |
1S neuron, seqment A5, animal 3, cell d306013 |
| GSM6936296 |
1S neuron, seqment A7, animal 3, cell d306014 |
| GSM6936297 |
1S neuron, seqment A6, animal 3, cell d306015 |
| GSM6936298 |
1S neuron, seqment A8, animal 3, cell d306016 |
| GSM6936299 |
1S neuron, seqment A8, animal 3, cell d306017 |
| GSM6936300 |
1S neuron, seqment A1, animal 3, cell d306018 |
| GSM6936301 |
1S neuron, seqment T3, animal 3, cell d306019 |
| GSM6936302 |
1S neuron, seqment T3, animal 3, cell d306020 |
| GSM6936303 |
1B neuron, seqment A1, animal 4, cell d306021 |
| GSM6936304 |
1B neuron, seqment T3, animal 4, cell d306022 |
| GSM6936305 |
1B neuron, seqment A1, animal 4, cell d306023 |
| GSM6936306 |
1B neuron, seqment A2, animal 4, cell d306024 |
| GSM6936307 |
1B neuron, seqment A3, animal 4, cell d306025 |
| GSM6936308 |
1B neuron, seqment A8, animal 4, cell d306026 |
| GSM6936309 |
1B neuron, seqment A8, animal 4, cell d306027 |
| GSM6936310 |
1B neuron, seqment A2, animal 4, cell d306028 |
| GSM6936311 |
1B neuron, seqment A5, animal 4, cell d306029 |
| GSM6936312 |
1B neuron, seqment A6, animal 4, cell d306030 |
| GSM6936313 |
1S neuron, seqment A6, animal 7, cell d306065 |
| GSM6936314 |
1S neuron, seqment A7, animal 7, cell d306066 |
| GSM6936315 |
1S neuron, seqment A5, animal 7, cell d306067 |
| GSM6936316 |
1S neuron, seqment A4, animal 7, cell d306068 |
| GSM6936317 |
1S neuron, seqment A3, animal 7, cell d306069 |
| GSM6936318 |
1S neuron, seqment A3, animal 7, cell d306070 |
| GSM6936319 |
1S neuron, seqment A1, animal 7, cell d306071 |
| GSM6936320 |
1S neuron, seqment A1, animal 7, cell d306072 |
| GSM6936321 |
1S neuron, seqment A2, animal 7, cell d306073 |
| GSM6936322 |
1S neuron, seqment A6, animal 7, cell d306074 |
| GSM6936323 |
1S neuron, seqment A8, animal 7, cell d306075 |
| GSM6936324 |
1S neuron, seqment A8, animal 7, cell d306076 |
| GSM6936325 |
1S neuron, seqment A3, animal 7, cell d306077 |
| GSM6936326 |
1S neuron, seqment A2, animal 7, cell d306078 |
| GSM6936327 |
1B neuron, seqment A1, animal 8, cell d306079 |
| GSM6936328 |
1B neuron, seqment A7, animal 8, cell d306080 |
| GSM6936329 |
1B neuron, seqment A4, animal 8, cell d306089 |
| GSM6936330 |
1B neuron, seqment A8, animal 8, cell d306090 |
| GSM6936331 |
1B neuron, seqment A3, animal 8, cell d306091 |
| GSM6936332 |
1B neuron, seqment A6, animal 8, cell d306092 |
| GSM6936333 |
1B neuron, seqment A5, animal 8, cell d306093 |
| GSM6936334 |
1B neuron, seqment A6, animal 8, cell d306095 |
| GSM6936335 |
1B neuron, seqment A3, animal 8, cell d306096 |
| GSM6936336 |
M4 muscle cell, segment A4, animal 1, cell d270002 |
| GSM6936337 |
M4 muscle cell, segment A5, animal 3, cell d270019 |
| GSM6936338 |
M4 muscle cell, segment A4, animal 3, cell d270020 |
| GSM6936339 |
M1 muscle cell, segment A4, animal 3, cell d270021 |
| GSM6936340 |
M1 muscle cell, segment A4, animal 1, cell d270025 |
| GSM6936341 |
M1 muscle cell, segment A2, animal 4, cell d270033 |
| GSM6936342 |
M1 muscle cell, segment A4, animal 6, cell d270039 |
| GSM6936343 |
M4 muscle cell, segment A4, animal 9, cell d270056 |
| GSM6936344 |
M4 muscle cell, segment A3, animal 9, cell d270057 |
| GSM6936345 |
M4 muscle cell, segment A2, animal 9, cell d270058 |
| GSM6936346 |
M4 muscle cell, segment A4, animal 10, cell d270070 |
| GSM6936347 |
M1 muscle cell, segment A2, animal 10, cell d270071 |
| GSM6936348 |
M4 muscle cell, segment A3, animal 10, cell d270072 |
| GSM6936349 |
M4 muscle cell, segment A2, animal 10, cell d270073 |
| GSM6936350 |
M1 muscle cell, segment A4, animal 10, cell d270074 |
| GSM6936351 |
M4 muscle cell, segment A4, animal 11, cell d270083 |
| GSM6936352 |
M4 muscle cell, segment A2, animal 11, cell d270085 |
| GSM6936353 |
M1 muscle cell, segment A3, animal 11, cell d270086 |
| GSM6936354 |
M4 muscle cell, segment A5, animal 11, cell d270087 |
| GSM6936355 |
M4 muscle cell, segment A2, animal 5, cell d306033 |
| GSM6936356 |
M1 muscle cell, segment A3, animal 5, cell d306034 |
| GSM6936357 |
M1 muscle cell, segment A4, animal 5, cell d306035 |
| GSM6936358 |
M4 muscle cell, segment A5, animal 5, cell d306036 |
| GSM6936359 |
M1 muscle cell, segment A2, animal 5, cell d306037 |
| GSM6936360 |
M1 muscle cell, segment A2, animal 5, cell d306038 |
| GSM6936361 |
M4 muscle cell, segment A5, animal 5, cell d306039 |
| GSM6936362 |
M1 muscle cell, segment A4, animal 5, cell d306040 |
| GSM6936363 |
M4 muscle cell, segment A2, animal 5, cell d306041 |
| GSM6936364 |
M4 muscle cell, segment A3, animal 5, cell d306042 |
| GSM6936365 |
M1 muscle cell, segment A3, animal 5, cell d306043 |
| GSM6936366 |
M4 muscle cell, segment A4, animal 5, cell d306044 |
| GSM6936367 |
M4 muscle cell, segment A5, animal 5, cell d306045 |
| GSM6936368 |
M4 muscle cell, segment A4, animal 6, cell d306046 |
| GSM6936369 |
M4 muscle cell, segment A4, animal 6, cell d306047 |
| GSM6936370 |
M4 muscle cell, segment A5, animal 6, cell d306048 |
| GSM6936371 |
M1 muscle cell, segment A4, animal 8, cell d306081 |
| GSM6936372 |
M4 muscle cell, segment A2, animal 8, cell d306082 |
| GSM6936373 |
M4 muscle cell, segment A4, animal 8, cell d306083 |
| GSM6936374 |
M1 muscle cell, segment A3, animal 8, cell d306084 |
| GSM6936375 |
M4 muscle cell, segment A4, animal 8, cell d306085 |
| GSM6936376 |
M1 muscle cell, segment A1, animal 8, cell d306086 |
| GSM6936377 |
M4 muscle cell, segment A2, animal 8, cell d306087 |
| GSM6936378 |
M1 muscle cell, segment A2, animal 8, cell d306088 |
|
| Relations |
| BioProject |
PRJNA924323 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE222976_1b1sm_intCt.txt.gz |
2.0 Mb |
(ftp)(http) |
TXT |
| GSE222976_1b1sm_isoform_intCt.txt.gz |
3.9 Mb |
(ftp)(http) |
TXT |
| GSE222976_1b1sm_isoform_l2tpm.txt.gz |
7.1 Mb |
(ftp)(http) |
TXT |
| GSE222976_1b1sm_l2tpm.txt.gz |
5.1 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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