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| Status |
Public on Oct 23, 2023 |
| Title |
The Myc binding partner Spt5 is essential for brain tumor growth in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The transcription factor SPT5 physically interacts with MYC oncoproteins and is essential for the full transcriptional activation of MYC targets in cultured cells. Here, we use Drosophila to show that SPT5 is also required for MYC-dependent processes in vivo. Depletion of SPT5 strongly impairs the growth of a model neuronal tumor and substantially extends survival of tumor-bearing animals. This beneficial effect is also observed when SPT5 is knocked down systemically after tumor initiation, highlighting SPT5 is a potential drug target in human oncology.
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| Overall design |
brat and/or Spt5 were knocked down in type II neuroblasts from larval stages on, using the genotypes: wor-GAL4 ase-GAL80 UAS-mCD8::GFP [UAS-brat-IR] [UAS-Spt5-IR]
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| Contributor(s) |
Ogunleye A, Hofstetter-Hadry J, Wolf E, Raabe T, Gallant P |
| Citation(s) |
37935464 |
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| Submission date |
Dec 05, 2022 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Martin Eilers |
| Organization name |
University of Wuerzburg
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| Department |
Chair for Biochemistry and Molecular Biology
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| Lab |
Martin Eilers
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| Street address |
Am Hubland
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| City |
Wuerzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA908816 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE220110_all_counts.tsv.gz |
217.1 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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