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| Status |
Public on Nov 29, 2024 |
| Title |
Impact of Vitamin-D3 on the gene expression profile of mouse colon |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Vitamin-D3 (VitD3) exhibits pleiotropic effects in host’s physiology by acting on several organs. To mechanistically understand how VitD3 influences the colon physiology we interrogated the gene expression profile of mouse colon in response to high or low VitD3 bioavailability. VitD3 tissue bioavailability was modulated in mice with the use of diets supplemented with different concentrations of VitD3 (0, 2 or 10 IU/g) and the use of Gc knockout mouse strain in where VitD3 bioavailability is enhanced.
Vitamin-D3 (VitD3) exhibits pleiotropic effects in host’s physiology by acting on several organs. To mechanistically understand how VitD3 influences the colon physiology we interrogated the gene expression profile of mouse colon in response to high or low VitD3 bioavailability. VitD3 tissue bioavailability was modulated in mice with the use of diets supplemented with different concentrations of VitD3 (0, 2 or 10 IU/g) and the use of Gc knockout mouse strain in where VitD3 bioavailability is enhanced.
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| Overall design |
RNA will be extracted from 5 biological replicates (5 individual mice) per condition (20 samples in total). Male mice (8 ½ weeks old) were fed with the following diets 3 ½ weeks :1)C57BL/6J/WT mice that were fed with standard VitD3 diet (2 IU/g) for 3 ½ weeks (n=5), 2) C57BL/6J/WT mice that were fed with high VitD3 diet (10 IU/g) for 3 ½ weeks (n=5), 3) Gc KO mice that were fed with standard VitD3 diet (2 IU/g) for 3 ½ weeks (n=5), 4) Gc KO mice that were fed with VitD3 deficient diet (10 IU/g) for 3 ½ weeks (n=5)
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| Web link |
https://www.science.org/doi/10.1126/science.adh7954?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed
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| Contributor(s) |
Giampazolias E, Reis e Sousa C |
| Citation(s) |
38662827 |
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| Submission date |
Dec 02, 2022 |
| Last update date |
Nov 29, 2024 |
| Contact name |
Probir Chakravarty |
| E-mail(s) |
[email protected]
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| Organization name |
Francis Crick Institute
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| Street address |
1 Midland Road
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| City |
London |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA907729 |
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