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| Status |
Public on Jul 18, 2023 |
| Title |
αKG treatment prevent maturation and stimulate proliferation of neonatal cardiomyocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In the heart, metabolic profile switches from glycolysis to fatty acid oxidation (FAO) rapidly after birth accompanied by chromatin reconfiguration, cell cycle exit and cardiomyocyte (CM) maturation, suggesting a synergistic rewiring of transcriptional networks regulated by epigenetic mechanisms in accordance with environmental and metabolic signals. To gain a better insight into the interconnection between epigenetic processes and metabolic pathways during cardiac development, maturation and heart regeneration, we induced a metabolic reprogramming by depleting CPT1b, a crucial enzyme for FAO, specifically in CM. Cpt1b inactivation led to cardiomegaly and attenuated cardiac damage in response to myocardial injury primarily attributed to augmented CM proliferation and enhanced resistance to ischemic injury.
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| |
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| Overall design |
Transcriptome profiles of P0-1 neonatal cardiomyocyte treated with DMSO and aKG for 96 hours
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| |
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| Contributor(s) |
Li X, Guenther S, Kuenne C, Braun T |
| Citation(s) |
37758950 |
| |
| Submission date |
Nov 03, 2022 |
| Last update date |
Oct 17, 2023 |
| Contact name |
Carsten Kuenne |
| E-mail(s) |
[email protected]
|
| Organization name |
Max Planck Institute for Heart and Lung Research
|
| Department |
Bioinformatics
|
| Street address |
Ludwigstrasse 43
|
| City |
Bad Nauheim |
| ZIP/Postal code |
61231 |
| Country |
Germany |
| |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA897885 |
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