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| Status |
Public on Oct 31, 2023 |
| Title |
Gene expression in Drosophila melanogaster in response to bacterial infection |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this study was to generate a high-quality dataset of gene expression in Drosophila melanogaster 3 hours after infection with Gram-positive (M luteus) or Gram-negative (E coli) bacteria.
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| Overall design |
RNAseq on polyA-selected RNA extracted from whole male adult flies either uninjected or 3 hours after injection with 50nl of either: PBS (vehicle control), E coli suspended in PBS (OD600=1), M luteus suspended in PBS (OD600=1). Flies were kept at 29° after injection on our standard lab diet (10% yeast, 8% fructose, 2% polenta, 0.8% agar, supplemented with nipagin and propionic acid).
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| Contributor(s) |
Dionne MS |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 18, 2022 |
| Last update date |
Oct 31, 2023 |
| Contact name |
Marc Dionne |
| E-mail(s) |
[email protected]
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| Organization name |
Imperial College London
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| Department |
Life Sciences
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| Street address |
Level 0, Flowers Building
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| City |
London |
| ZIP/Postal code |
SW7 2AZ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (20)
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| GSM6657652 |
D melanogaster, uninjected, 3 hours, biol rep 1 |
| GSM6657653 |
D melanogaster, uninjected, 3 hours, biol rep 2 |
| GSM6657654 |
D melanogaster, uninjected, 3 hours, biol rep 3 |
| GSM6657655 |
D melanogaster, uninjected, 3 hours, biol rep 4 |
| GSM6657656 |
D melanogaster, uninjected, 3 hours, biol rep 5 |
| GSM6657657 |
D melanogaster, PBS-injected, 3 hours, biol rep 1 |
| GSM6657658 |
D melanogaster, PBS-injected, 3 hours, biol rep 2 |
| GSM6657659 |
D melanogaster, PBS-injected, 3 hours, biol rep 3 |
| GSM6657660 |
D melanogaster, PBS-injected, 3 hours, biol rep 4 |
| GSM6657661 |
D melanogaster, PBS-injected, 3 hours, biol rep 5 |
| GSM6657662 |
D melanogaster, E coli injected, 3 hours, biol rep 1 |
| GSM6657663 |
D melanogaster, E coli injected, 3 hours, biol rep 2 |
| GSM6657664 |
D melanogaster, E coli injected, 3 hours, biol rep 3 |
| GSM6657665 |
D melanogaster, E coli injected, 3 hours, biol rep 4 |
| GSM6657666 |
D melanogaster, E coli injected, 3 hours, biol rep 5 |
| GSM6657667 |
D melanogaster, M luteus injected, 3 hours, biol rep 1 |
| GSM6657668 |
D melanogaster, M luteus injected, 3 hours, biol rep 2 |
| GSM6657669 |
D melanogaster, M luteus injected, 3 hours, biol rep 3 |
| GSM6657670 |
D melanogaster, M luteus injected, 3 hours, biol rep 4 |
| GSM6657671 |
D melanogaster, M luteus injected, 3 hours, biol rep 5 |
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| Relations |
| BioProject |
PRJNA891752 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE216042_wt_gene-level_TPMs_6.33.csv.gz |
1.9 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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