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| Status |
Public on Apr 28, 2023 |
| Title |
Heat shock transcription factors demonstrate a distinct mode of interaction with mitotic chromosomes |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
A large number of transcription factors have been shown to bind and interact with mitotic chromosomes, which may promote the efficient reactivation of transcriptional programs following cell division. Although the DNA-binding domain (DBD) contributes strongly to TF behavior, the mitotic behaviors of TFs from the same DBD family may vary. To define the mechanisms governing TF behavior during mitosis in mouse embryonic stem cells, we examined two related TFs: Heat Shock Factor 1 and 2 (HSF1 and HSF2). We found that HSF2 maintains site-specific binding genome-wide during mitosis, whereas HSF1 binding is somewhat decreased. Surprisingly, live-cell imaging shows that both factors appear excluded from mitotic chromosomes to the same degree, and are similarly more dynamic in mitosis than in interphase. Exclusion from mitotic DNA is not due to extrinsic factors like nuclear import and export mechanisms. Rather, we found that the HSF DBDs can coat mitotic chromosomes, and that HSF2 DBD is able to establish site-specific binding. These data further confirm that site-specific binding and chromosome coating are independent properties, and that for some TFs, mitotic behavior is largely determined by the non-DBD regions.
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| Overall design |
Cleavage Under Target and Tagmentation (CUT&Tag) for transcription factors Sox2, HSF1, and HSF2 in asynchronous and mitotic JM8 mouse embryonic stem cells.
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| Web link |
https://doi.org/10.1093/nar/gkad304
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| Contributor(s) |
Price RM, Budzyński MA, Shen J, Mitchell JE, Kwan JJ, Teves SS |
| Citation(s) |
37114996 |
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| Submission date |
Sep 26, 2022 |
| Last update date |
Sep 07, 2023 |
| Contact name |
Sheila Teves |
| E-mail(s) |
[email protected]
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| Organization name |
Life Science Institute
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| Department |
Biochemistry
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| Lab |
Teves Lab
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| Street address |
2350 Health Sciences Mall
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| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6T 1Ze |
| Country |
Canada |
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| Platforms (1) |
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| Samples (38)
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| GSM7118130 |
JM8, HSF1, mitosis, rep2 |
| GSM7118131 |
JM8, HSF2, asynchronous, rep1 |
| GSM7118132 |
JM8, HSF2, asynchronous, rep2 |
| GSM7118133 |
JM8, HSF2, mitosis, rep1 |
| GSM7118134 |
JM8, HSF2, mitosis, rep2 |
| GSM7118135 |
JM8, Sox2, asynchronous, rep1 |
| GSM7118136 |
JM8, Sox2, asynchronous, rep2 |
| GSM7118137 |
JM8, Sox2, mitosis, rep1 |
| GSM7118138 |
JM8, Sox2, mitosis, rep2 |
| GSM7118139 |
JM8, HSF2-OE, asynchronous, rep1 |
| GSM7118140 |
JM8, HSF2-OE, asynchronous, rep2 |
| GSM7118141 |
JM8, HSF2-OE, mitosis, rep1 |
| GSM7118142 |
JM8, HSF2-OE, mitosis, rep2 |
| GSM7118143 |
JM8, HSF2 Full_Length, HA tag, asynchronous, rep1 |
| GSM7118144 |
JM8, HSF2 Full_Length, HA_tag, asynchronous, rep2 |
| GSM7118145 |
JM8, HSF2 Full_Length, HA tag, mitosis, rep1 |
| GSM7118146 |
JM8, HSF2 Full_Length, HA tag, mitosis, rep2 |
| GSM7118147 |
JM8, HSF2 DBD, HA tag, asynchronous, rep1 |
| GSM7118148 |
JM8, HSF2 DBD, HA tag, asynchronous, rep2 |
| GSM7118149 |
JM8, HSF2 DBD, HA tag, mitosis, rep1 |
| GSM7118150 |
JM8, HSF2 DBD, HA tag, mitosis, rep2 |
| GSM7118151 |
JM8, HSF2, asynchronous, 10% WT cells, rep1 |
| GSM7118152 |
JM8, HSF2, asynchronous, 10% WT cells, rep2 |
| GSM7118153 |
JM8, HSF2, asynchronous, 5% WT cells, rep1 |
| GSM7118154 |
JM8, HSF2, asynchronous, 5% WT cells, rep2 |
| GSM7118155 |
JM8, HSF2 KO, asynchronous, rep1 |
| GSM7118156 |
JM8, HSF2 KO, asynchronous, rep2 |
| GSM7118157 |
JM8, HSF2 KO, mitosis, rep1 |
| GSM7118158 |
JM8, HSF2 KO, mitosis, rep2 |
| GSM7118159 |
JM8, HA ctrl, asynchronous, rep1 |
| GSM7118160 |
JM8, HA ctrl, asynchronous, rep2 |
| GSM7118161 |
JM8, IgG, asynchronous, rep1 |
| GSM7118162 |
JM8, IgG, asynchronous, rep2 |
| GSM7118163 |
JM8, IgG, mitosis, rep1 |
| GSM7118164 |
JM8, IgG, mitosis, rep2 |
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| Relations |
| BioProject |
PRJNA884392 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE214219_HSF2-mean-scores.tab.gz |
169.5 Kb |
(ftp)(http) |
TAB |
| GSE214219_JM8-H1-scores.tab.gz |
47.6 Kb |
(ftp)(http) |
TAB |
| GSE214219_JM8-H1_peaks.bed.gz |
47.6 Kb |
(ftp)(http) |
BED |
| GSE214219_JM8-H2-peaks.bed.gz |
58.7 Kb |
(ftp)(http) |
BED |
| GSE214219_JM8-Sox2-scores.tab.gz |
354.3 Kb |
(ftp)(http) |
TAB |
| GSE214219_RAW.tar |
1.6 Gb |
(http)(custom) |
TAR (of BW) |
| GSE214219_SOX2_peaks_mm10.bed.gz |
111.6 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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