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| Status |
Public on Jun 01, 2010 |
| Title |
Transcriptome analysis of highly metastatic cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
A prediction of peritoneal recurrence is of significance using metastasis-related biomarker. This work describes a combined analysis of proteome and transcriptome data for biomarker discovery in highly metastatic cell line. We used nano-flow liquid chromatography (LC) linear ion trap time-of-flight mass spectrometry (LIT-TOF MS) and cDNA microarray to identify specific protein differentially expressed between a highly metastatic stomach cancer cell line MKN-45-P and its parental cell line MKN-45. In total, 240 proteins were found to be expressed between the two cell lines. Of these, 75 proteins (31%) and 49 proteins (20%) were only identified from MKN-45-P and MKN-45 respectively. An mRNA expression of 1533 genes was up-regulated in MKN-45-P compared with MKN-45. No close correlation was found between proteomic and transcriptomic analysis. Interestingly, 4 of 240 proteins (1.6%) were involved in the up-regulated mRNA expression.
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| Overall design |
Comparison analysis of gene expression between highly metastatic gastric cancer cell line MKN-45-P and its parental cell line MKN-45.
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| |
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| Contributor(s) |
Ohshima K, Hatakeyama K |
| Citation(s) |
21548097 |
| |
| Submission date |
Apr 13, 2010 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Keiichi HATAKEYAMA |
| E-mail(s) |
[email protected]
|
| Organization name |
Shizuoka Cancer Center Research Institute
|
| Street address |
1007 Shimonagakubo
|
| City |
Nagaizumi-cho |
| State/province |
Sunto-gun, Shizuoka |
| ZIP/Postal code |
411-8777 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA126177 |
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