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| Status |
Public on May 31, 2023 |
| Title |
Gene expression profiling of N87 and OE19 gastric cancer cell lines grown in 3D or 2D condition |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A great deal of evidence indicates that solid and hematological tumors are driven and maintained by a minor cell compartment with stem cell properties. In this context, gastric cancer stem cells (GCSCs) are implicated in tumor aggressiveness, invasion, metastasis and therapy resistance. HER2 amplification/overexpression (HER2+) is found in about 20% of GCs. Although the anti-HER2 antibody trastuzumab has improved the outcomes of patients with HER2+ advanced disease, its benefits are usually transitory because of high HER2 heterogeneity and frequent co-existence of other oncogenic drivers associated with primary/acquired resistance. To identify potential drivers of trastuzumab resistance in GCSCs, we compare the gene expression profile of OE19 and N87 HER2+ GC cell lines cultured as gastrospheres, peculiar 3D cell cultures that allow detecting the in vitro enrichment of CSCs, versus their matched adherent cell (2D) counterparts. Comparison of the transcriptomic profiles of HER2+ GC cells cultured in 2D versus 3D conditions evidenced a significant enrichment of Fatty Acid Synthase (FASN) transcript among the genes upmodulated in 3D cell cultures. We found that the pharmacological blockade of HER2 and FASN targets CSCs and decreases tumor growth of HER2-positive GC models
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| Overall design |
Gastrospheres were derived from OE19 and N87 HER2+ GC cell lines. Gene expression profiles of these cells were compared with the profiles of the same cells cultured in adherent conditions (2D). (n=3 biological replicates)
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| Contributor(s) |
Pupa S, Castagnoli L, De Cecco L, Dugo M |
| Citation(s) |
36753044 |
| |
| Submission date |
Aug 31, 2022 |
| Last update date |
Jun 01, 2023 |
| Contact name |
Matteo Dugo |
| E-mail(s) |
[email protected]
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| Organization name |
IRCCS Ospedale San Raffaele
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| Department |
Department of Medical Oncology
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| Street address |
via Olgettina 60
|
| City |
Milan |
| ZIP/Postal code |
20132 |
| Country |
Italy |
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| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
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| Samples (12)
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| GSM6532861 |
N87 gastric cancer cell line grown in 2D condition (replicate 1) |
| GSM6532862 |
N87 gastric cancer cell line grown in 2D condition (replicate 2) |
| GSM6532863 |
N87 gastric cancer cell line grown in 2D condition (replicate 3) |
| GSM6532864 |
N87 gastric cancer cell line grown in 3D condition (replicate 1) |
| GSM6532865 |
N87 gastric cancer cell line grown in 3D condition (replicate 2) |
| GSM6532866 |
N87 gastric cancer cell line grown in 3D condition (replicate 3) |
| GSM6532867 |
OE19 gastric cancer cell line grown in 2D condition (replicate 1) |
| GSM6532868 |
OE19 gastric cancer cell line grown in 2D condition (replicate 2) |
| GSM6532869 |
OE19 gastric cancer cell line grown in 2D condition (replicate 3) |
| GSM6532870 |
OE19 gastric cancer cell line grown in 3D condition (replicate 1) |
| GSM6532871 |
OE19 gastric cancer cell line grown in 3D condition (replicate 2) |
| GSM6532872 |
OE19 gastric cancer cell line grown in 3D condition (replicate 3) |
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| Relations |
| BioProject |
PRJNA875439 |
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