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Series GSE211435 Query DataSets for GSE211435
Status Public on Nov 07, 2023
Title Expression data from human pancreatic cancer cell lines and mouse orthotopic tumors generated using human PDAC cell lines replete and depleted for RBFOX2
Platform organism Homo sapiens
Sample organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by array
Summary RBFOX2 is an RNA binding protein that directs alternative splicing. In this study, we characterized RBFOX2-mediated alternative splicing in pancreatic cancer (PDAC)
In this dataset, we assayed gene-level and exon-level expression differences in pancreatic cancer cell lines replete and depleted for RBFOX2 expression and in 8 pairs of orthotopic pancreas tumors and related liver metastases generated from human 4039 or Panc1 cell lines replete or depleted for RBFOX2.
 
Overall design 3 human PDAC cell line pairs replete (NTC) and depleted (KD) for RBFOX2 with no biological replicates were analyzed for differential gene expression and exon usage on a population level, comparing KD vs.NTC. Gene-level and exon-level data for human genes expressed in the population of orthotopic pancreas tumors generated using RBFOX2 depleted human 4039 or Panc1 cell lines was compared with gene-level and exon-level data for human genes expressed in the population of RBFOX2 replete orthotopic pancreas tumors generated using 4039 or Panc1 cells (KD vs. NTC). Array data from matched liver metastases and from two unmodified cell lines with low RBFOX2 expression were included in the normalization.
 
Contributor(s) Mann KM
Citation(s) 38114498
Submission date Aug 17, 2022
Last update date Jan 03, 2024
Contact name Karen M Mann
Organization name Moffitt Cancer Center
Street address 12902 Magnolia Drive
City Tampa
State/province FL
ZIP/Postal code 33612
Country USA
 
Platforms (1)
GPL23126 [Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version]
Samples (24)
GSM6470022 4039 cell line with RBFOX2 expression
GSM6470023 4039 cell line with RBFOX2 depletion
GSM6470024 Panc1 cell line with RBFOX2 expression
Relations
BioProject PRJNA870318

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Supplementary file Size Download File type/resource
GSE211435_RAW.tar 604.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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