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| Status |
Public on Mar 25, 2010 |
| Title |
TGFb dependent Treg conversion and the inhibition of conversion by IL6 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of CD4+ cells activated with anti-CD3 and cultured in the presence of IL2. The effects of TGFb and IL6 on Treg conversion were analyzed to discover methods of inhibiting Treg conversion and/or Treg functional inhibition. CD4+ T-cells were enriched from lymph nodes of Foxp3-GFP mice and cultured under the under the following conditions: 1) anti-CD3+IL2; 2) anti-CD3+IL2+TGFβ; 3) anti-CD3+IL2+TGFβ+IL6; and 4) anti-CD3+IL2+IL6. On day 4 cells were collected, stained with anti-CD4-PE, and sorted into CD4+FOXP3- and CD4+FOXP3+ populations (>95% purity). For each treatment condition T-effector cells and Tregs were labeled independently (either cy3 or cy5) and hybridized together for a two-color array experiment. Each treatment condition had dye swapped replicates, and a minimum of 3 replicate in total.
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| Overall design |
Two color experiment. Four conditions : 1) anti-CD3+IL2, 3 samples 2) anti-CD3+IL2+TGFβ, 3 samples 3) anti-CD3+IL2+TGFβ+IL6, 4 samples 4) anti-CD3+IL2+IL6, 3 samples
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| Contributor(s) |
Hoelzinger DB, Lustgarten J |
| Citation(s) |
20483762 |
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| Submission date |
Mar 23, 2010 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Cheryl E Myers |
| E-mail(s) |
[email protected]
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| Phone |
480-301-4056
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| Organization name |
Mayo Clinic College of Medicine
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| Department |
Immunology
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| Lab |
Cohen
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| Street address |
13400 E Shea Blvd
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| City |
Scottsdale |
| State/province |
AZ |
| ZIP/Postal code |
85259 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA125781 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21027_RAW.tar |
184.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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