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| Status |
Public on Jun 15, 2023 |
| Title |
Separation of transcriptional repressor and activator functions in HDAC3 [RNA-Seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The histone deacetylase HDAC3 is associated with the NCoR/SMRT co-repressor complex and its canonical function is in transcriptional repression, but it can also activate transcription. Here we show that the repressor and activator functions of HDAC3 can be genetically separated in Drosophila. A lysine substitution in the N-terminus (K26A) disrupts its catalytic activity and activator function, whereas a combination of substitutions (HEBI) abrogating the interaction with SMRTER enhance repressor activity beyond wild-type in the early embryo. We conclude that the critical functions of HDAC3 in embryo development involve catalytic-dependent gene activation and non-enzymatic repression by several mechanisms, including tethering of loci to the nuclear periphery.
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| Overall design |
Analysis of gene expression by RNA-seq from HDAC3 shRNA, shRNA-resistant rescueWT, rescueY303F, rescueK26A, rescueHEBI and Control (tub-Gal4/+) embryos in 4 replicates.
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| Contributor(s) |
Tang M, Regadas I, Belikov S, Mannervik M |
| Citation(s) |
37455638 |
| |
| Submission date |
Jun 16, 2022 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Mattias Mannervik |
| E-mail(s) |
[email protected]
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| Organization name |
Stockholm University
|
| Street address |
Svante Arrhenius väg 20C
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| City |
Stockholm |
| ZIP/Postal code |
10691 |
| Country |
Sweden |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE206249 |
Separation of transcriptional repressor and activator functions in HDAC3 |
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| Relations |
| BioProject |
PRJNA849808 |
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