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| Status |
Public on Sep 30, 2022 |
| Title |
Early-life exposure to tobacco smoke alters airway signaling pathways in D. melanogaster and later mortality in D. melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the effect of cigarette smoke exposure on gene expression in airway epithelial cells of Canton S Drosophila melanogaster larvae, we isolated the airways of cigarette smoke exposed larvae and air controls.
We then performed gene expression profiling analysis using data obtained from RNA-seq of smoke-exposed males, smoke-exposed females, air-control males and air-control females.
For each group 4 biological replicates were prepared, representing 40-50 larval airways.
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| Overall design |
Comparative gene expression analysis of RNA-seq data for isolated trachea of smoke exposed and air controls of Canton S Drosophila melanogaster larvae, separated into males and females
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| Contributor(s) |
Sirocko K, Angstmann H, Papenmeier S, Wagner C, Spohn M, Indenbirken D, Kovacevic D, Ehrhardt B, Hammer B, Svanes C, Rabe KF, Roeder T, Uliczka K, Krauss-Etschmann S |
| Citation(s) |
35780997 |
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| Submission date |
May 30, 2022 |
| Last update date |
Jan 04, 2023 |
| Contact name |
Susanne Krauss-Etschmann |
| E-mail(s) |
[email protected]
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| Organization name |
Resarch Center Borstel, Leibniz Lung Center
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| Department |
Early Life Origins of Chronic Lung Diseases
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| Street address |
Parkallee 1-44
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| City |
Borstel |
| ZIP/Postal code |
23845 |
| Country |
Germany |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (16)
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| GSM6205104 |
D.melanogaster trachea, cigarette smoke exposed female, rep. 1 |
| GSM6205105 |
D.melanogaster trachea, cigarette smoke exposed female, rep. 2 |
| GSM6205106 |
D.melanogaster trachea, cigarette smoke exposed female, rep. 3 |
| GSM6205107 |
D.melanogaster trachea, cigarette smoke exposed female, rep. 4 |
| GSM6205108 |
D.melanogaster trachea, air control female, rep. 1 |
| GSM6205109 |
D.melanogaster trachea, air control female, rep. 2 |
| GSM6205110 |
D.melanogaster trachea, air control female, rep. 3 |
| GSM6205111 |
D.melanogaster trachea, air control female, rep. 4 |
| GSM6205112 |
D.melanogaster trachea, cigarette smoke exposed male, rep. 1 |
| GSM6205113 |
D.melanogaster trachea, cigarette smoke exposed male, rep. 2 |
| GSM6205114 |
D.melanogaster trachea, cigarette smoke exposed male, rep. 3 |
| GSM6205115 |
D.melanogaster trachea, cigarette smoke exposed male, rep. 4 |
| GSM6205116 |
D.melanogaster trachea, air control male, rep. 1 |
| GSM6205117 |
D.melanogaster trachea, air control male, rep. 2 |
| GSM6205118 |
D.melanogaster trachea, air control male, rep. 3 |
| GSM6205119 |
D.melanogaster trachea, air control male, rep. 4 |
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| Relations |
| BioProject |
PRJNA843700 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE205093_female_counts_norm.tsv.gz |
659.0 Kb |
(ftp)(http) |
TSV |
| GSE205093_male_counts_norm.tsv.gz |
654.4 Kb |
(ftp)(http) |
TSV |
| GSE205093_regulated_genes_smoke_vs._air_-_females.xlsx |
2.3 Mb |
(ftp)(http) |
XLSX |
| GSE205093_regulated_genes_smoke_vs._air_-_males.xlsx |
2.4 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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