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| Status |
Public on Dec 27, 2022 |
| Title |
Recycling of parental histones preserves the epigenetic landscape during embryonic development (RNA-seq) |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Epigenetic inheritance during DNA replication requires an orchestrated assembly of nucleosomes from parental and newly synthesized histones. We analyzed Drosophila HisC mutant embryos harboring a deletion of all canonical histone genes, in which nucleosome assembly relies on parental histones from cell cycle 14 onwards. Lack of new histone synthesis and parental histone recycling leads to more accessible chromatin and reduced nucleosome occupancy. This leads to upregulated and spurious transcription, whereas the control of the developmental transcriptional program is partially maintained. Importantly, the genomic positions of modified parental H2A, H2B, and H3 are restored during DNA replication. Therefore, the results suggest that parental histones with active or repressive marks are recycled to preserve the epigenetic landscape during DNA replication in vivo.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for wild type embryos (w1118) and mutant embryos (Df(2L)HisC) lacking newly synthesized histones.
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| Contributor(s) |
Günesdogan U, Jäckle H, Li X, Dovgusha O, Mühlen D |
| Citation(s) |
36724233 |
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| Submission date |
May 20, 2022 |
| Last update date |
Mar 02, 2023 |
| Contact name |
Ufuk Günesdogan |
| E-mail(s) |
[email protected]
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| Organization name |
University of Göttingen
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| Street address |
Justus-von-Liebig Weg 11
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| City |
Göttingen |
| ZIP/Postal code |
37077 |
| Country |
Germany |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (23)
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| GSM6172745 |
HisC, 6.5-7h, rep1 |
| GSM6172746 |
w1118, 3.5-4h, rep1 |
| GSM6172747 |
w1118, 4.5-5h, rep1 |
| GSM6172748 |
w1118, 5.5-6h, rep1 |
| GSM6172749 |
w1118, 6.5-7h, rep1 |
| GSM6172750 |
HisC, 3.5-4h, rep2 |
| GSM6172751 |
HisC, 4.5-5h, rep2 |
| GSM6172752 |
HisC, 5.5-6h, rep2 |
| GSM6172753 |
HisC, 6.5-7h, rep2 |
| GSM6172754 |
w1118, 3.5-4h, rep2 |
| GSM6172755 |
w1118, 4.5-5h, rep2 |
| GSM6172756 |
w1118, 5.5-6h, rep2 |
| GSM6172757 |
w1118, 6.5-7h, rep2 |
| GSM6172758 |
HisC, 3.5-4h, rep3 |
| GSM6172759 |
HisC, 5.5-6h, rep3 |
| GSM6172760 |
HisC, 6.5-7h, rep3 |
| GSM6172761 |
w1118, 3.5-4h, rep3 |
| GSM6172762 |
w1118, 4.5-5h, rep3 |
| GSM6172763 |
w1118, 5.5-6h, rep3 |
| GSM6172764 |
w1118, 6.5-7h, rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE203478 |
Recycling of parental histones preserves the epigenetic landscape during embryonic development |
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| Relations |
| BioProject |
PRJNA840917 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE203471_Dmel-RNA-seq_counts_TPM.geo.txt.gz |
700.1 Kb |
(ftp)(http) |
TXT |
| GSE203471_Dmel-RNA-seq_counts_normalized_by_sizefactor.geo.txt.gz |
2.0 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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