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| Status |
Public on May 20, 2022 |
| Title |
Genomic characterization of nub[1] wing tissue |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Profiled chromatin accessibility and gene expression in the wing imaginal discs of Drosophila melanogaster, in wild type and nub[1] animals. We demonstrate using RNA-seq that expression of both nub and pdm2 paralogs are reduced in the nub[1] condition, and that this allele contains a previously unannotated deletion within the intergenic region between of nub and pdm2 using ATAC-seq that corresponds to a wing enhancer element.
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| Overall design |
Using ATAC-seq and RNA-seq to profile chromatin accessibility and gene expression in nub/pdm2 mutant wing tissue. ChIP-seq using an rotund-GFP in 3rd instar wing imaginal discs
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| Contributor(s) |
Ryan L, Richard MS |
| Citation(s) |
35853455 |
| |
| Submission date |
May 17, 2022 |
| Last update date |
Aug 19, 2022 |
| Contact name |
Ryan Loker |
| E-mail(s) |
[email protected]
|
| Phone |
(212) 305-2111
|
| Organization name |
Columbia University
|
| Street address |
3227 Broadway
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA839032 |
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