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| Status |
Public on May 16, 2022 |
| Title |
Single-cell RNA-Sequencing maps the hematopoietic landscape in naked mole-rats [unfractionated PB] |
| Organism |
Heterocephalus glaber |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Naked mole-rats are a mammalian model organism of exceptional longevity. We mapped the hematopoietic hierarchy and used functional characterizations to define a purified stem and progenitor cell (HSPC) compartment similar to mouse LIN-/Sca-1+/Kit+ HSPCs or human LIN-/CD34+/CD38lo HSPCs.
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| Overall design |
Naked mole-rat peripheral blood was extracted, RBC-lysed and submitted to scRNA-Seq as unfractionated BM. The mRNA libraries were aligned to either the FRAMA bone marrow transcriptome assembly from our publication.
Note: Raw data represents only gene expresion (GEX). Raw data for ADT not included.
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| Contributor(s) |
Emmrich S |
| Citation(s) |
34596321, 38762584 |
| |
| Submission date |
May 12, 2022 |
| Last update date |
Jun 06, 2024 |
| Contact name |
Stephan Emmrich |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Rochester
|
| Street address |
213 Hutchison Hall, River Campus
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14627 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL30845 |
Illumina NovaSeq 6000 (Heterocephalus glaber) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE185724 |
Single-cell RNA-Sequencing maps the hematopoietic landscape in naked mole-rats. |
|
| Relations |
| BioProject |
PRJNA842730 |
| SRA |
SRP384257 |
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