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| Status |
Public on Jul 02, 2023 |
| Title |
H3K27me3 ChIP-sequencing of control and α-KG treated neonatal primary cardiomyocytes |
| Organism |
Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
purpose:To compare the enrichment of H3K27me3 on genes after treatment of control and α-KG. Method: Primary cardiomyocytes were treated with control (PBS) and α-KG for 24 h and precipitated with H3K27me3 antibody, then, the precipitated sequences were analysed by next generation RNA-seq. Results: There are significant differences between control and α-KG group. Conclusion:H3K27me3 ChIP-seq was used for analysing the enrichment of H3K27me3 on genes.
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| Overall design |
PBS treated primary cardiomyocytes and α-KG treated primary cardiomyocytes
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| Contributor(s) |
Shi Y, Tian M, Tang L |
| Citation missing |
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| BioProject |
PRJNA826668 |
| |
| Submission date |
May 04, 2022 |
| Last update date |
Jul 03, 2023 |
| Contact name |
Yu Shi |
| E-mail(s) |
[email protected]
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| Phone |
15692776868
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| Organization name |
Military Medical University
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| Department |
Cardiology
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| Lab |
cardiovascular disease institute
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| Street address |
10 Changjiang Branch Road
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| City |
Chongqing |
| State/province |
Chongqing |
| ZIP/Postal code |
400042 |
| Country |
China |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (6)
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