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| Status |
Public on Oct 13, 2010 |
| Title |
Bifidobacterium bifidum BF-1 Suppresses Helicobacter pylori-Induced genes in Human Epithelial Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We have shown that pre-incubation with Bifidobacterium bifidum (B. bifidum) strain BF-1, a probiotic strain known to improve H. pylori-associated gastritis, suppresses the induction of IL-8 by the pathogen. To investigate how BF-1 affects gene expression in H. pylori-infected cells, we performed microarray analysis to assess gene expression in epithelial cells, which had been pre-incubated with BF-1 and infected with H. pylori.
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| Overall design |
Four-condition experiment. Biological replicates with a dye-exchange experiment. Biological replicates with a longer incubation time experiment.
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| Contributor(s) |
Shirasawa Y, Ishikawa F, Shibahara-Sone H |
| Citation(s) |
20854986 |
| |
| Submission date |
Feb 03, 2010 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Yukio Shirasawa |
| E-mail(s) |
[email protected]
|
| Phone |
81-42-577-8992
|
| Fax |
81-42-577-8934
|
| Organization name |
Yakult Honsha Co., Ltd.
|
| Department |
Central Institute for microbiological research
|
| Lab |
Analysis center
|
| Street address |
1796 Yaho
|
| City |
Kunitachi |
| State/province |
Tokyo |
| ZIP/Postal code |
186-8650 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
|
| Samples (12)
|
| GSM506117 |
control (ch1) vs. infected 4 h (ch2) |
| GSM506118 |
BF-1+non-infected (ch1) vs BF-1+infected 4 h (ch2) |
| GSM506119 |
infected (ch1) vs BF-1+infected 4 h (ch2) |
| GSM506120 |
control (ch1) vs. BF-1 4 h (ch2) |
| GSM506121 |
control (ch1) vs. infected dye-exchage (ch2) |
| GSM506122 |
BF-1+non-infected (ch1) vs BF-1+infected dye-exchange (ch2) |
| GSM506123 |
infected (ch1) vs BF-1+infected dye-exchange (ch2) |
| GSM506124 |
control (ch1) vs. BF-1 dye-exchange (ch2) |
| GSM506125 |
control (ch1) vs. infected 6 h (ch2) |
| GSM506126 |
BF-1+non-infected (ch1) vs BF-1+infected 6 h (ch2) |
| GSM506127 |
infected (ch1) vs BF-1+infected 6 h (ch2) |
| GSM506128 |
control (ch1) vs. BF-1 6 h (ch2) |
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| Relations |
| BioProject |
PRJNA125635 |
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