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| Status |
Public on Jan 14, 2010 |
| Title |
Characterization of the mid-foregut transcriptome identifies genes regulated during lung bud induction. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To identify genes expressed during initiation of lung organogenesis, we generated transcriptional profiles of the prospective lung region of the mouse foregut (mid-foregut) microdissected from embryos at three developmental stages between embryonic day 8.5 (E8.5) and E9.5. This period spans from lung specification of foregut cells to the emergence of the primary lung buds. We identified a number of known and novel genes that are temporally regulated as the lung bud forms. Genes that regulate transcription, including DNA binding factors, co-factors, and chromatin remodeling genes, are the main functional groups that change during lung bud formation. Members of key developmental transcription and growth factor families, not previously described to participate in lung organogenesis, are expressed in the mid-foregut during lung bud induction. These studies also show early expression in the mid-foregut of genes that participate in later stages of lung development. This characterization of the mid-foregut transcriptome provides new insights into molecular events leading to lung organogenesis.
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| Overall design |
Three samples (developmental stages), three biological replicates (5-10 pooled mid-foreguts)
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| Contributor(s) |
Millien G, Beane J, Lenburg M, Tsao P, Lu J, Spira A, Ramirez MI |
| Citation(s) |
18023262 |
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| Submission date |
Jan 13, 2010 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Maria Isabel Ramirez |
| E-mail(s) |
[email protected]
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| Organization name |
Boston University School of Medicine
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| Department |
Medicine
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| Lab |
Pulmonary Center
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| Street address |
72 E. Concord St. E603
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (9)
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| GSM496702 |
16-20 somites mid-foregut, biological rep1 |
| GSM496703 |
16-20 somites mid-foregut, biological rep2 |
| GSM496704 |
16-20 somites mid-foregut, biological rep3 |
| GSM496705 |
21-25 somites mid-foregut, biological rep1 |
| GSM496706 |
21-25 somites mid-foregut, biological rep2 |
| GSM496707 |
21-25 somites mid-foregut, biological rep3 |
| GSM496708 |
26-30 somites mid-foregut, biological rep1 |
| GSM496709 |
26-30 somites mid-foregut, biological rep2 |
| GSM496710 |
26-30 somites mid-foregut, biological rep3 |
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| Relations |
| BioProject |
PRJNA121967 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19873_RAW.tar |
21.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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