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| Status |
Public on Apr 27, 2022 |
| Title |
Environmental Induced Epigenetic Transgenerational Inheritance of Pathology: Systems Epigenetics in Disease Etiology and Generational Toxicology |
| Organism |
Rattus norvegicus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Several epigenome-wide association studies (EWAS) have been shown to identify epigenetic alterations (i.e., epimutations) associated with diseases. The sperm epimutations potentially involved in the transgenerational inheritance of specific pathologies have been identified. Transgenerational sperm epimutations associated with kidney, prostate, puberty, testis, obesity, and multiple pathologies have been identified for a variety of environmental toxicants including dioxin, plastics, pesticides, glyphosate, methoxychlor, atrazine, and jet fuel. The transgenerational sperm epimutations for exposure and disease-specific epimutations have been identified in these EWAS studies. The current study used the information from these previous toxicant-induced epigenetic transgenerational inheritance EWAS rat studies and adds a comparable control group, rats that have not been exposed to any particular toxicant. Two additional control groups were collected and are presented here.
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| Overall design |
From embryonic day E8 to E14 the F0 generation gestating females were administered daily intraperitoneal injections of dimethyl sulfoxide (DMSO) (vehicle control) with an equal volume of sesame oil (Sigma) to prevent irritation at the injection site. The offspring F1, F2 and F3 generations were all aged to 70-90 days of age and bred within the generation and within the control lineage. The F3 generation is the first without a direct exposure to the vehicle control and is thus comparable to the transgenerational generation in the exposure lineages. All the animals were aged to 1 year and euthanized for pathology and sperm collected for epigenetic analyses. No sibling or cousin breeding was used to prevent any inbreeding artifacts in the control lineage. An MeDIP-seq approach was used to identify DMRs associated with specific transgenerational diseases.
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| Contributor(s) |
Beck D, Nilsson EE, Maamar MB, Skinner MK |
| Citation(s) |
35440735 |
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| Submission date |
Mar 11, 2022 |
| Last update date |
Apr 27, 2022 |
| Contact name |
Michael K Skinner |
| E-mail(s) |
[email protected]
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| Organization name |
WSU
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| Department |
SBS
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| Street address |
Abelson 507
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| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99163 |
| Country |
USA |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (89)
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| Relations |
| BioProject |
PRJNA815328 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE198452_control.results.csv.gz |
493.3 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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