|
| Status |
Public on May 01, 2024 |
| Title |
Genome-wide bisulfite sequencing (GWBS) and RNA-seq in hippocampus of Neil1- and Neil2 deficient mice |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
We report the bisulfite sequencing and RNA-seq in hippocampus of Neil deficient adult mice. By genome-wide bisulphite sequencing (GWBS) analysis, NEIL depletion mice (including Neil1-/-, Neil2-/- and Neil1-/- Neil2-/-) consistently displayed global hypo-methylation of 5mC in base resolution in CG context, as well as in gene feature regions including transcription start sites (TSS) in CHG and CHH context by compared to wild type. RNA-seq reveals differentially expressed genes are mainly overlapped with differential methylation. A subset of overlap genes is enriched in synaptic function. Together, NEIL1 and NEIL2 functionally promote DNA methylation and impact gene expression.
|
| |
|
| Overall design |
Examination of genome-wide 5mC and 5hmC in hippocampus of Neil deficient adult mice compared to wild type; investigation the correlation between methylaiton and gene expression.
|
| |
|
| Contributor(s) |
Yang M, Scheffler K, Bjoras M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 06, 2022 |
| Last update date |
May 01, 2024 |
| Contact name |
Mingyi Yang |
| E-mail(s) |
[email protected]
|
| Phone |
004746613633
|
| Organization name |
Oslo University Hospital
|
| Department |
Department of Microbiology, Rikshospitalet
|
| Street address |
Sognsvannsveien 20
|
| City |
Oslo |
| ZIP/Postal code |
0372 |
| Country |
Norway |
| |
|
| Platforms (1) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA813323 |
Less...
More...