|
| Status |
Public on Feb 23, 2022 |
| Title |
Newly defined human hematopoietic stem cell populations |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Transcriptomic profiling (RNA-seq) of primitive human progenitor populations CD34+CD38-CD45RA-CD90-EPCR-; hereafter CD90-EPCR- MPP.
|
| |
|
| Overall design |
Purpose: To characterize the transcriptomic profile of the human CD90-EPCR- MPP progenitor population.
Methods: Cells were obtained from human cord-blood and flow-sorted using the surface antigens described. 5 CD90-EPCR- biological replicates were used to extract total RNA and quality was verified (RIN >8).
Libraries were prepared using 20-25 ng/sample of starting RNA. All samples were sequenced two independent times.
Conclusions: This study represents the analysis of transcriptomes from a human progenitor population, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles of other human blood stem/progenitor populations.
|
| |
|
| Contributor(s) |
Anjos-Afonso F, Buettner F, Bonnet D |
| Citation(s) |
35440586 |
| |
| Submission date |
Feb 20, 2022 |
| Last update date |
May 30, 2022 |
| Contact name |
Florian Buettner |
| Organization name |
DKFZ
|
| Department |
DKTK Frankfurt
|
| Street address |
Theodor-Stern-Kai 7
|
| City |
Frankfurt |
| State/province |
Germany |
| ZIP/Postal code |
60590 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA808797 |
Less...
More...