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| Status |
Public on Feb 17, 2022 |
| Title |
Gene expression of central amygdala of mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goals of this study are to screen out the changes of gene expression profile in response to high fat diet, an mRNA-seq was performed in central amygdala
Methods: 6-week-old male wild-type mice were fed with high fat diet (HFD, containing 45% fat) or normal chow diet (ND) for 5 months. And then their central amygdala tissues were removed and total RNA was extracted to perform RNA sequencing.
Results:The KEGG pathway analysis showed that genes related to neuroactive ligand-receptor interaction and calcium signaling pathway were dramatically upregulated after HFD
Conclusions: Our study represents the first detailed analysis of central amygdalar transcriptomes of mice subjected to high fat diet, with biologic replicates, generated by RNA-seq technology. Our results show genes related to neuroactive ligand-receptor interaction and calcium signaling pathway were dramatically upregulated after high fat diet feeding,which hint at the importance of ion channel in central amygdala after high fat feeding.
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| Overall design |
Cental amygdalar mRNA profiles of mice challenged with 20-week normal chow diet or high fat diet
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| Contributor(s) |
Ma H, Zhu Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Feb 14, 2022 |
| Last update date |
Feb 23, 2022 |
| Contact name |
Ma Huan |
| E-mail(s) |
[email protected]
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| Phone |
15123809290
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| Organization name |
Daping Hospital,Army Medical University
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| Street address |
Daping Street
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| City |
Chongqing |
| ZIP/Postal code |
400042 |
| Country |
China |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA806964 |
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