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| Status |
Public on Jun 01, 2024 |
| Title |
RNA-seq profiling of wild type and Trim33-/- splenic dendritic cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The homeostatic control mechanism of dendritic cells (DCs), including pDCs, cDC1s and cDC2s, is not fully elucidated. Transcriptome profiling of wildtype and TRIM33 conditional knockout mice revealed a key role of TRIM33 in maintaining the homeostasis of all DC subsets.
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| Overall design |
Splenic pDCs (CD11c+ SiglecH+), cDC1s (CD11c+ SiglecH- MHC II+ CD24+ CD172a-) and cDC2s (CD11c+ SiglecH- MHC II+ CD24- CD172a+) of 6 to 8-week old Trim33fl/fl (WT) or Trim33fl/fl Itgax-Cre (KO) mice were isolated from spleen by depleting non-DC lineage with a homemade antibody cocktail and fluorescent activated cell sorting. Total RNA was extracted with the TRIzol reagent. 2 - 3 biological replicates were prepared for each cell subset of each genotype. Transcriptome was profiled by RNA-seq at the Beijing Genomics Institution (BGI) on the BGISEQ-500 platform to generate 50 base-pair single end reads.
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| Contributor(s) |
Shen X, Wu L |
| Citation(s) |
38822080 |
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| Submission date |
Jan 06, 2022 |
| Last update date |
Jun 02, 2024 |
| Contact name |
Xiangyi Shen |
| E-mail(s) |
[email protected]
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| Organization name |
Tsinghua University
|
| Department |
The Institute for Immunology, School of Medicine
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| Street address |
D303 Zhengyutong Medical Building, Tsinghua University
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| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA795190 |
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