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| Status |
Public on Jan 03, 2022 |
| Title |
RNA-sequencing analysis of mouse epithelial NMuMG cells with Cas9 or UGCG knock out |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the gene expression in mouse NMuMG cells with or without UGCG depletion using RNA sequencing. After data analysis, the 11218 transcripts were identified. Among these transcripts, the TGF-β signaling target genes and EMT marker genes were significantly upregulated in UGCG depleted NMuMG cells. Then we used these genes and mouse TGF-β or EMT gene signatures to do the gene set enrichment analysis and showed the negative correlation of UGCG and TGF-β signaling or EMT in NMuMG cells.
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| Overall design |
RNA profiles of mouse NMuMG cells with Cas9 (as the control group) and UGCG knock out. Each group contains three biological replicates.
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| Contributor(s) |
Zhang J, ten Dijke P, Mei H, Agaser CC |
| Citation(s) |
36504224 |
| |
| Submission date |
Dec 27, 2021 |
| Last update date |
Feb 08, 2023 |
| Contact name |
Hailiang Mei |
| E-mail(s) |
[email protected]
|
| Organization name |
Leiden University Medical Center
|
| Department |
Sequencing Analysis Support Core
|
| Street address |
Einthovenweg 20
|
| City |
Leiden |
| ZIP/Postal code |
2333 ZC |
| Country |
Netherlands |
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| Platforms (1) |
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| Samples (6)
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| GSM5761200 |
NMuMG cells expressing Cas9 (Replicate #1) |
| GSM5761201 |
NMuMG cells expressing Cas9 (Replicate #2) |
| GSM5761202 |
NMuMG cells expressing Cas9 (Replicate #3) |
| GSM5761203 |
NMuMG cells with UGCG knock out (Replicate #1) |
| GSM5761204 |
NMuMG cells with UGCG knock out (Replicate #2) |
| GSM5761205 |
NMuMG cells with UGCG knock out (Replicate #3) |
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| Relations |
| BioProject |
PRJNA792612 |
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