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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 31, 2010 |
| Title |
Cbfb/Runx1-repression independent blockage of differentiation and accumulation of Csf2rb expressing cells by Cbfb-MYH11 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
It is known that CBFB-MYH11, the fusion gene generated by inversion of chromosome 16 in human acute myeloid leukemia, is causative for oncogenic transformation. However, the mechanism by which CBFB-MYH11 initiates leukemogenesis is not clear. Previously published reports showed that CBFB-MYH11 dominantly inhibits RUNX1 and CBFB, and such inhibition has been suggested as the mechanism for leukemogenesis. However, knockin mice expressing Cbfb-MYH11 (Cbfb+/MYH11) showed defects in primitive hematopoiesis not seen in Cbfb null (Cbfb-/-) embryos indicating that Cbfb-MYH11 has repression independent activities as well.
To identify gene expression changes associated with this novel activity, we compared the gene expression profile in the blood cells of Cbfb+/MYH11 and Cbfb-/- embryonic day 12.5 (E12.5) embryos with that of their wildtype littermates.
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| Overall design |
Cbfb-MYH11 chimeras were mated to C57/Bl6 females to generate Cbfb+/MYH11 (Cbfb+/MYH11) and Cbfb+/+ (WT) embryos. Cbfb+/- x Cbfb+/- matings were used to generate Cbfb+/+ (Cbfb+/+) and Cbfb-/- (Cbfb-/-) embryos. Blood from 8-10 E12.5 embryos of the same genotype was pooled, and RNA was isolated, labeled, and hybridized to Affymetrix Genechip mouse microarray (430 2.0) chips. 4 chips were used for both the Cbfb+/MYH11 and littermate control samples. 3 chips were used for the Cbfb-/- samples and littermate control samples.
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| Contributor(s) |
Hyde RK, Kamikubo Y, Anderson S, Kirby M, Alemu L, Zhao L, Liu PP |
| Citation(s) |
20007544 |
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| Submission date |
Nov 25, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Christopher Pan |
| E-mail(s) |
[email protected]
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| Organization name |
NIH
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| Department |
NHGRI
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| Street address |
Rm 5228, Bldg 50, South Dr.
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (14)
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| GSM475933 |
Cbfb+/MYH11, technical replicate 4 |
| GSM475934 |
WT, technical replicate 1 |
| GSM475935 |
WT, technical replicate 2 |
| GSM475936 |
WT, technical replicate 3 |
| GSM475937 |
WT, technical replicate 4 |
| GSM475938 |
Cbfb-/-, technical replicate 1 |
| GSM475939 |
Cbfb-/-, technical replicate 2 |
| GSM475940 |
Cbfb-/-, technical replicate 3 |
| GSM475941 |
Cbfb+/+, technical replicate 1 |
| GSM475942 |
Cbfb+/+, technical replicate 2 |
| GSM475943 |
Cbfb+/+, technical replicate 3 |
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| Relations |
| BioProject |
PRJNA120677 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19194_RAW.tar |
48.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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