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| Status |
Public on Jan 01, 2022 |
| Title |
Genome-wide maps of chromatin state in bone marrow cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
GFI1 is a transcription factor and was implicated in the development of MDS. Reduced expression of GFI1 or presence of the GFI1-36N variant leads to epigenetic changes. Using GFI1-36S, -36N -KD, NUP98-HOXD13-tg mice and curcumin (a natural histone acetyltransferase inhibitor (HATi)), we now demonstrate that expansion of GFI1-36N or –KD, NUP98-HODXD13 leukemic cells can be delayed. Curcumin treatment significantly reduced AML progression in GFI1-36N or -KD mice and prolonged AML-free survival. Of note, curcumin treatment had no effect in GFI1-36S, NUP98-HODXD13 expressing mice. On a molecular level, curcumin treatment negatively affected open chromatin structure in the GFI1-36N or -KD haematopoietic cells but not GFI1-36S cells. Taken together, our study thus identified a therapeutic role for curcumin treatment in the treatment of AML patients (homo or heterozygous for GFI1-36N or reduced GFI1 expression) and possibly improved therapy outcome.
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| Overall design |
Examination of different mice mode histone odifcation changes in bone marrow cells.
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| Contributor(s) |
Xie X, Frank D, Patnana PK, Schutte J, Al-Matary Y, Wei L, Dugas M, Varghese J, Nimmagadda SC, khandanpour C |
| Citation(s) |
35008835 |
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| Submission date |
Dec 15, 2021 |
| Last update date |
Jan 20, 2022 |
| Contact name |
Xiaoqing Xie |
| E-mail(s) |
[email protected]
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| Organization name |
Hematology, Oncology and Pneumology
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| Department |
Department of Medicine A
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| Street address |
Albert-Schweitzer-Campus 1
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| City |
Muenster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| GSM5736754 |
bone marrow cells 36N no curcumin treatment |
| GSM5736755 |
bone marrow cells 36N curcumin treatment |
| GSM5736756 |
bone marrow cells 36S no curcumin treatment |
| GSM5736757 |
bone marrow cells 36S curcumin treatment |
| GSM5736758 |
bone marrow cells KD no curcumin treatment |
| GSM5736759 |
bone marrow cells KD curcumin treatment |
| GSM5736760 |
bone marrow cells 36S no curcumin treatment input control |
| GSM5736761 |
bone marrow cells 36N no curcumin treatment rabbit IgG |
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| Relations |
| BioProject |
PRJNA789232 |
| SRA |
SRP351332 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE190974_RAW.tar |
3.2 Gb |
(http)(custom) |
TAR (of BW, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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