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| Status |
Public on Feb 01, 2022 |
| Title |
Transcriptome analysis of LT-HSC isolated from WT and CD169DTR/+ mice 5 days after a 2 Gy-Total Body Irradiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
A 2 Gy-total body irradiation (TBI) of WT mice induces a second wave of ROS and a second wave of apoptosis in hematopoietic stem cells starting from 6 days after irradiation. In CD169DTR/+ mice, which are depleted in CD169+ resident macrophages after a diphtheria toxin injection, these two waves do not occur and a total recovery of the LT-HSC pool is observed, contrarily to the LT-HSC pool from WT mice, which is decreased. Our goal is to understand why these secondary waves of apoptosis and ROS occur in LT-HSC from irradiated WT mice and not in LT-HSC from irradiated mice depleted in resident macrophages, allowing a recovery of the LT-HSC pool.
We used microarrays to compare the global gene expression in LT-HSC isolated from WT and CD169DTR/+ mice 5 days after irradiation, i.e. just before both secondary waves of ROS and apoptosis occurred in WT mice.
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| Overall design |
Two distinct experiments have been performed to obtain two replicates for each condition (i.e. for both mouse strains, irradiated or not).
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| Contributor(s) |
MORENO S |
| Citation(s) |
35100346 |
| |
| Submission date |
Nov 23, 2021 |
| Last update date |
May 03, 2022 |
| Contact name |
Stephanie G. MORENO |
| E-mail(s) |
[email protected]
|
| Organization name |
CEA
|
| Department |
DRF / iRCM
|
| Lab |
LRTS
|
| Street address |
18 route du Panorama
|
| City |
Fontenay-aux-roses |
| State/province |
92 |
| ZIP/Postal code |
92265 |
| Country |
France |
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| Platforms (1) |
| GPL20258 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) CDF version] |
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| Samples (8)
|
| GSM5702320 |
LT-HSC from non-irradiated WT mouse_rep1 |
| GSM5702321 |
LT-HSC from non-irradiated WT mouse_rep2 |
| GSM5702322 |
LT-HSC from 2 Gy-irradiated WT mouse_rep1 |
| GSM5702323 |
LT-HSC from 2 Gy-irradiated WT mouse_rep2 |
| GSM5702324 |
LT-HSC from non-irradiated CD169DTR/+ mouse_rep1 |
| GSM5702325 |
LT-HSC from non-irradiated CD169DTR/+ mouse_rep2 |
| GSM5702326 |
LT-HSC from 2 Gy-irradiated CD169DTR/+ mouse_rep1 |
| GSM5702327 |
LT-HSC from 2 Gy-irradiated CD169DTR/+ mouse_rep2 |
|
| Relations |
| BioProject |
PRJNA783058 |
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