|
| Status |
Public on Oct 08, 2021 |
| Title |
The C. burnetii T4SS effector protein AnkG hijacks the 7SK small nuclear riboprotein complex for reprogramming host cell transcription |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
In this study, we analyzed the molecular activity of the anti-apoptotic T4SS effector protein AnkG (CBU0781) to understand how C. burnetii manipulates host cell viability. We demonstrate by RNA-immunoprecipitation that AnkG binds to the host cell 7SK snRNA, an important regulator of the positive transcription elongation factor P-TEFb. Consistent with the documented function of released P-TEFb in RNA Pol II pause release, RNA sequencing experiments confirmed AnkG-mediated transcriptional reprogramming and showed that expression of genes involved in apoptosis, trafficking, and transcription is influenced by AnkG.
|
| |
|
| Overall design |
RNA-Seq of HEK293T cells transfected with either GFP-NLS-AnkG or GFP alone and RIP-Seq of HEK293T cells transfected GFP-NLS-AnkG
|
| |
|
| Contributor(s) |
Cordsmeier A, Jeninga M, Petter M, Lührmann A |
| Citation(s) |
35134097 |
| |
| Submission date |
Oct 06, 2021 |
| Last update date |
Feb 18, 2022 |
| Contact name |
Michaela Petter |
| E-mail(s) |
[email protected]
|
| Phone |
+4991318522177
|
| Organization name |
Univeritätsklinikum Erlangen
|
| Department |
Mirkobiologisches Institut
|
| Street address |
Wassertrumstr. 3-5
|
| City |
Erlangen |
| ZIP/Postal code |
91054 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA769091 |
| SRA |
SRP340251 |
Less...
More...