|
| Status |
Public on Sep 30, 2010 |
| Title |
Effect of AAF overexpression on the leaves before bolting |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of leaves from 3-week old transgenic plants overexpressing AAF, which is a senescence-associated gene. Overexpression of AAF promoted leaf senescence and accumulation of reactive oxygen species (ROS) in transgenic plants. Results provide insight in the role of AAF in ROS homeostasis and the function in leaf senescence.
|
| |
|
| Overall design |
3-week-old plants of Col-0 (wild type, as control) and AAF-OX (overexpression line) were grown in long day condition (16hr light/8hr dark). Rosette leaves of Col-0 and AAF-OX were used for total RNA extraction and cDNA synthesis. cDNA labeling, array hybridization, and scanning followed standard Affymetrix expression array protocol. Two independent sets of microarray analyses were performed in this study.
|
| |
|
| Contributor(s) |
Chen G, Wang LK |
| Citation(s) |
21940719 |
| |
| Submission date |
Sep 30, 2009 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Kevin Long-Chi WANG |
| E-mail(s) |
[email protected]
|
| Phone |
+886227871181
|
| Fax |
+886227827954
|
| Organization name |
Academia Sinica
|
| Department |
Institute of Plant and Microbial Biology
|
| Street address |
128 Section 2, Academia Road
|
| City |
Taipei |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA118001 |
Less...
More...