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| Status |
Public on Aug 09, 2010 |
| Title |
Transcriptome changes triggered by Hyaloperonospora parasitica arabidopsis Noco2 in WT and wrky72 mutants at 96 hpt |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
Expression profiling by high throughput sequencing
|
| Summary |
Functional characterization of AtWRKY72 using Arabidopsis T-DNA insertion lines showed that this gene is important for basal defense to root-knot nematode (RKN) and Hyaloperonospora parasitica arabidopsis (Hpa), but not several tested R gene-mediated defenses.To profile transcriptional reprogramming associated with AtWRKY72-dependent basal defense we used Affymetrix ATH1 GeneChips representing ~24,000 Arabidopsis genes. Three independent biological replicates were performed with Col-0, wrky72-1 and wrky72-2 plants at 96 hpt with HpaNoco2 or mock treatment. Using a false discovery rate of less than 0.05 we identified for each of these three lines genes that showed significant transcriptional changes in response to HpaNoco2 compared to the mock-treated controls. Identification of downstream targets of WRKY72 in Arabidopsis by this microarray suggests that WRKY72 uses a unique signaling pathway that involves AP2/ERF TFs independent of the ethylene signaling pathway.
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| Overall design |
We intended to identify Arabidopsis genes that are regulated in WRKY72-dependent manner in response to Hyaloperonospora parasitica arabidopsis (Hpa) at 96 hpt. For this, we used Col-0 and two wrky72 mutant lines and compared the differentially expressed genes with the hypothesis ‘genes that are specifically regulated in Col-0 but not in wrky72 mutants are WRKY72-dependent genes’.
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| Contributor(s) |
Bhattarai KK, Kaloshian I, Eulgem T |
| Citation(s) |
20409007 |
| |
| Submission date |
Sep 29, 2009 |
| Last update date |
Sep 03, 2021 |
| Contact name |
Kishor Kumar Bhattarai |
| E-mail(s) |
[email protected]
|
| Phone |
607-254-6424
|
| Organization name |
University of California
|
| Department |
Plant Pathology
|
| Lab |
Kaloshian
|
| Street address |
9000 University Avenue
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (18)
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA117987 |
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