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| Status |
Public on Feb 15, 2022 |
| Title |
Topical estrogen application promotes cutaneous wound healing in db/db female mice with type 2 diabetes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Female sex hormones are beneficial effects for wound healing. However, till date, whether topical estrogen application can promote cutaneous wound healing in diabetes remains unclear. Therefore, the present study aimed to validate the effect of topical estrogen application on cutaneous wound healing in a type 2 diabetes db/db mice model. In total, 22 db/db female mice with type 2 diabetes and eight C57BL/6J female mice were subjected to two full-thickness wound injuries. The mice were divided into the db/db, db/db + estrogen, db/db + vehicle, and wild type (WT) groups. Wound healing was assessed until day 14. The db/db group had a significantly high wound area ratio (wound area/initial wound area) on days 3–14 and a significantly low re-epithelialization ratio on days 7 and 14. Moreover, their angiogenesis ratio was significantly low on day 7 and high on day 14. In contrast, compared with the db/db group, the db/db + estrogen group had a significantly lower wound area ratio on days 1–14 and angiogenesis ratio on day 14, thereby indicating early withdrawal of new blood vessels, as well as a significantly higher re-epithelialization ratio on days 7 and 14 and Ym1+ M2 macrophage/macrophage ratio on day 7. Moreover, microarray analysis showed that the top 10 upregulated or downregulated genes in the db/db group were reversed by estrogen treatment, particularly on day 14, in comparison with the WT group. Thus, topical estrogen application reduced the wound area, promoted re-epithelialization and angiogenesis, and increased the number of M2 macrophages in mice with type 2 diabetes. Furthermore, it improved the differential regulation of genes in db/db mice. Therefore, such treatment can enhance cutaneous wound healing in female mice with type 2 diabetes.
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| Overall design |
Comparison in eight groups: diabetic group (db/db), a topical estrogen wound treatment diabetic group (db/db+E), vehicle wound treatment diabetic group (db/db+vehicle) and wild-type group (WT) on Day7 and Day14 after wound, respectively.There are two biological replicates for each conditions.
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| Contributor(s) |
Mukai K, Horike S, Meguro-Horike M, Nakajima Y, Iswara A, Nakatani T |
| Citation(s) |
35271602 |
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| Submission date |
Aug 27, 2021 |
| Last update date |
May 17, 2022 |
| Contact name |
Shin-ichi Horike |
| E-mail(s) |
[email protected]
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| Phone |
+81-76-265-2775
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| Organization name |
Kanazawa University
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| Department |
Research Center for Experimental Modeling of Human Disease
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| Lab |
HORIKE Lab
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| Street address |
13-1 Takaramachi
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| City |
Kanazawa |
| State/province |
Ishikawa |
| ZIP/Postal code |
920-0934 |
| Country |
Japan |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA758274 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE182906_RAW.tar |
34.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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