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| Status |
Public on Dec 19, 2021 |
| Title |
Differential gene expression analysis identified determinants of cell fate plasticity during radiation-induced regeneration in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used here a combination of lineage tracing, FACS and genome-wide RNAseq to identify two key changes that accompany hinge-to-pouch fate change: (1) repression of hinge determinants and (2) upregulation of ribosome biogenesis.
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| Overall design |
We performed a genome-wide analysis of gene expression changes during regeneration of the Drosophila larval wing disc. By dissociating the discs into single cells and using Fluorescence-Activated Cell Sorting we monitored the hinge versus the rest of the disc, to identify genes that are differentially expressed in the two cell populations during regeneration and fate change.
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| Contributor(s) |
Ledru M, Clark C, Brown J, Verghese S, Ferrara S, Goodspeed A, Su TT |
| Citation(s) |
34990447 |
| |
| Submission date |
Aug 25, 2021 |
| Last update date |
Jan 28, 2022 |
| Contact name |
Andrew Goodspeed |
| Organization name |
University of Colorado Anschutz Medical Campus
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| Street address |
12801 E 17th Ave, Mail Stop 8303
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA757764 |
| SRA |
SRP334116 |
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