|
| Status |
Public on Aug 23, 2021 |
| Title |
Distinct Functional Properties of Perinatal vs. Adult Adipose Progenitors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We previously identified functional distinct sub-populations in adult male gWAT Pdgfrb+ cells utilizing the MuralChaser lineage tracking system and single-cell RNA-seq. Later in this related submission, we performed single-cell RNA-seqs on different developing stage of gWAT (P3, P7 and 5-week). We identified 4 sub-populations of Pdgfrb+ cells. Bulk-mRNA were conducted to these sub-populations in order to understand their transcriptomic profiles.
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| |
|
| Overall design |
Bulk mRNA-seq was done on freshly isolated Pdgfrb+ sub-populations (adipocyte precursor cells, fibro-inflammatory progenitors, mesothelial-like cells, smooth muscle cells) by FACS.
|
| |
|
| Contributor(s) |
Zhang Q, Gupta RK |
| Citation(s) |
35982290 |
| |
| Submission date |
Jul 30, 2021 |
| Last update date |
Aug 22, 2022 |
| Contact name |
Rana K Gupta |
| E-mail(s) |
[email protected]
|
| Organization name |
Duke University
|
| Department |
Duke Molecular Physiology Institute
|
| Street address |
Carmichael Building, 300 North Duke St.
|
| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27701 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA751103 |
| SRA |
SRP330635 |
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