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| Status |
Public on Sep 09, 2010 |
| Title |
Functional redundancy of iron acquisition systems is required for Proteus mirabilis pathogenesis during UTI |
| Organism |
Proteus mirabilis HI4320 |
| Experiment type |
Expression profiling by array
|
| Summary |
Proteus mirabilis is a primary cause of complicated urinary tract infections (UTI). Surprisingly, iron acquisition systems have been poorly characterized in this uropathogen despite the urinary tract being iron-limited. In this report the transcriptome of strain HI4320, cultured under iron limitation, was examined using microarray analysis. Of genes upregulated at least 2-fold, 45 were statistically significant and comprise 21 putative iron-regulated systems. Two of these systems, PMI0229-0239 and PMI2596-2605, are organized in operons and appear to encode siderophore biosynthesis genes.
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| Overall design |
Five microarrays comparing P. mirabilis HI4320 cultured in LB broth to P. mirabilis cultured in LB broth + 15 uM Desferal (an iron chelator) were analyzed. All five arrays are biological replicates; arrays #2 and 4 are dye swaps.
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| |
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| Contributor(s) |
Himpsl SD, Pearson MM, Mobley HL |
| Citation(s) |
20923418 |
| |
| Submission date |
Sep 09, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Melanie M Pearson |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Michigan Medical School
|
| Department |
Microbiology and Immunology
|
| Lab |
Melanie Pearson
|
| Street address |
1150 W Medical Center Dr
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-0620 |
| Country |
USA |
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| Platforms (1) |
| GPL9078 |
UMich_Proteus mirabilis_v1.0 3.7k array |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA119307 |
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