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| Status |
Public on Dec 06, 2021 |
| Title |
Whole transcriptome sequencing of HT22 immortalized mouse hippocampal cells treated with AA147 or veh for 16 h |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Define transcriptional remodeling associated with AA147 treatment (10uM) for 16 h in a mouse neuronal cell line.
Methods: A minimum of 27M PE100 reads were generated for triplicates of vehicle vs. AA147 treated HT22 cells. Alignment of reads was performed using DNAstar Lasergene SeqManPro to the mouse genome GRCm39 assembly. Read counts generated by ArrayStar 12.2 QSeq package. Differential expression analysis performed using DESeq2.
Results: We mapped between 27-32 M sequence reads per sample to the mouse genome and identified 16,473 transcripts with counts greater than 10. Of these, we identified 2,742 genes with significantly different expression between vehicle and AA147 treated cells.
Conclusions: We observed substantial upregulation of prolactin and glutathione transferase genes, and upregulation of NRF2-associated pathway upregulation.
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| Overall design |
Whole transcriptome RNA-Seq performed on HT22 mouse neuronal cells treated with vehicle or AA147 for 16 h.
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| Contributor(s) |
Rosarda JD, Wiseman RL |
| Citation(s) |
34797633 |
| |
| Submission date |
Jun 26, 2021 |
| Last update date |
Dec 06, 2021 |
| Contact name |
R. Luke Wiseman |
| Organization name |
The Scripps Research Institute
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| Department |
Dept. of Molecular Medicine
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| Street address |
10550 N Torrey Pines Rd
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| City |
La Jolla |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA741680 |
| SRA |
SRP325720 |
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